1,3-dimethylimidazolidin-2-one

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

No test method stated as study designed for notification of a substance under Japanese Law. Can be assigned Klimisch code 2 on the basis the study design is acceptable to a national chemical regulatory authority and the data are sufficiently well documented to show results are scientifically acceptable. Study design meets all the requirements of current OECD Guideline 305 except that the number fish used and whether a control group was used is not stated. Also there is no indication of whether any mortality was observed.

Qualifier:

according to guideline

Guideline:

other: Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substances (Japan 1973 Law. No. 117)

GLP compliance:

no

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: 2, 4, 6 weeks
- Sampling intervals/frequency for test medium samples: 3, 5, 8, 12, 15, 19, 22, 2629, 33, 36, 40 days
- Details on sample storage: samples analysed directly after preparation
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods): fish - measured for weight and length then extracted two times with chloroform after maceration with sand (10g) and anhydrous sodium sulfate (200g). The filtrate was then filtered under vacuum. Test medium - sodium chloride (30g/40ppm and 300g/400ppm sample) added to 100ml test medium and extracted 3 times with chloroform.

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION OF SPIKED SEDIMENT
Not applicable

PREPARATION OF SPIKED WATER
- Details of spiking: 1g test substance dissolved in 1 litre deionised water (1000ppm) then further diluted to 400ppm (level 1) and 40ppm (level 2). The diluted solutions were used in the flow through system with a dilution rate of 4ml.min (lower test level) and 800ml/min (higher test level)
- Controls: test medium prepared without the test substance
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none used, the substance is soluble in water
- Concentration of vehicle in final test solution: not applicable, vehicle not used

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Strain: n/a
- Source: not reported
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 10.4 cm (average)
- Weight at study initiation (mean and range, SD): 29.0g (average)
- Weight at termination (mean and range, SD):
-
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): 25°C
- Type and amount of food: not reported
- Feeding frequency: not reported
- Health during acclimation (any mortality observed): no mortality, all fish had normal appearance by visual observation

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Test temperature:

25°C

Dissolved oxygen:

6-8 mg/l

Salinity:

not applicable - freshwater study

Details on test conditions:

TEST SYSTEM
- Test vessel: 100-liter test aquaria
- Material: glass
- Type of flow-through (e.g. peristaltic or proportional diluter): continuous
- Renewal rate of test solution (frequency/flow rate): 4ml/min lower test level; 800 ml/min higher test level

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: not reported
- Light intensity: not reported

Nominal and measured concentrations:

Test medium (nominal): 0.2ppm and 2ppm
Test medium (measured): 2 weeks 1.86ppm; 4 weeks 1.85ppm; 6 weeks 1.86 (average concentration, high level)
Test medium (measured): 2 weeks 0.193ppm; 4 weeks 0.191ppm; 6 weeks 0.191 (average concentration, low level)

Reference substance (positive control):

no

Details on estimation of bioconcentration:

BASIS INFORMATION
- Results from residue study: yes
- Monitoring data: yes

BASIS FOR CALCULATION OF BCF
Detection limit for concentration of test substance in fish divided by the mean concentration of test substance in the test media for 6 weeks

Key result

Type:

BCF

Value:

< 0.37 dimensionless

Remarks on result:

other: no test substance was detected in fish

Details on kinetic parameters:

Not applicable - no test substance was detected in fish

Metabolites:

Not applicable - no test substance was detected in fish

Results with reference substance (positive control):

Not applicable - positive control not used

Details on results:

- Mortality of test organisms: no mortality
- Behavioural abnormalities: no behavioural abnormalities
- Observations on body length and weight: appearance normal from visual observation
- Mortality and/or behavioural abnormalities of control: no mortality or behavioural abnormalities reported
- Loss of test substance during test period: no significant loss
- Results with vehicle control: not applicable vehicle control not used

BCF = detection limit in fish/measured concentration in water

level 1 (2ppm): BCF = 0.68ppm/1.86ppm = 0.37

level 2 (0.2ppm): BCF = 0.68ppm/0.191 = 3.6

Validity criteria fulfilled:

yes

Conclusions:

The bioconcentration factor of the test substance is less than 0.37.

Description of key information

The bioconcentration factor of the test substance is less than 0.37.

Key value for chemical safety assessment

BCF (aquatic species):

0.37

Additional information

The substance has a very low bioconcentration factor and therefore has little or no propensity to bioaccumulate.