Chlorimuron ethyl

Toxicity to other above-ground organisms

Administrative data

Endpoint:

toxicity to other above-ground organisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Subdivision L - Hazard evaluation: Nontarget Insects, of the FIFRA Registration Guidelines US EPA, October 15, 1982.

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate

EC Number:

618-690-2

Cas Number:

90982-32-4

Molecular formula:

C15H15ClN4O6S

IUPAC Name:

ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate

Test material form:

solid

Details on test material:

- Purity: 92.5%

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

yes

Remarks:

Acetone

Test organisms

Test organisms (species):

other: Honey bees

Study design

Study type:

laboratory study

Remarks:

Single dermal application

Limit test:

no

Post exposure observation period:

48 h

Test conditions

Test temperature:

78°F

Photoperiod and lighting:

16 hours light and 8 hours dark photocycle

Nominal and measured concentrations:

Test A: 12.5 and 6.25 µg/bee
Test B: 12.5, 6.25, 3.125, and 1.5625 µg/bee

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LD50

Effect conc.:

> 12.5 other: µg/bee

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LD50: >12.5 µg/bee (Mortality)

Executive summary:

The test substance was evaluated for acute contact toxicity in honey bees (Apis mellifera L.). The test methods are consistent with the procedures specified in Subdivision L - Hazard Evaluation: Nontarget Insects, of the FIFRA Registration Guidelines US EPA, October 15, 1982.

Test bees were immobilized by confining them in 8 oz cylindrical plastic containers (11 cm diameter) in groups of approximately 100, and placing them in a freezer for four minutes.

Once immobilized, each bee received the appropriate dose in one milliliter of acetone, applied with a micropipette. Each bee was held by a wing with feather weight forceps, and the dose applied dorsally on the bee's thorax. The bee was then transferred to the appropriate test chamber for observation. Mortality responses were noted at the end of 48 hours.

In two separate tests, no mortality occurred at 12.5 µg/bee, the highest rate tested. Based on these findings, the acute contact LD50 was estimated to be greater than 12.5 µg/bee. The LD50 for the standard, carbaryl, approximated 1 µg/bee in each test.

The test substance was considered to be relatively nontoxic to the honey bee.