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EC number: 204-317-7
CAS number: 119-36-8
Methyl salicylate was one of 17 chemicals tested in an examination
of the LLNA assay for use in contact sensitization risk assessment
In a slightly midified LLNA protocol, test items were applied on
both surfaces of ears of 5 femaleCBA/J mice per group (25 µl/ear) for
four consecutive days (Days 1, 2, 3 and 4). MeS was tested at
concentrations of 0, 1, 2.5 and 5% in acetone. On Day 5, the cell
proliferation in the local lymph nodes was measured for each individual
mouse by incorporation of tritiated methyl thymidine ([3H]TdR).
The values obtained were used to calculate stimulation indices (SI).
A chemical was considered positive (sensitizer) in this assay if
exposure to at least one concentration resulted in a 2-fold or greater
increase in [3H]TdR, expressed as disintegrations per minute
(dpm) provided that this mean dpn value was statistically different from
vehicle-treated mice (p=< 0.01). Chemicals wee considered to be moderate
to strong sensitizers if the increase was >30-fold and weak to moderate
sensitiser if the increase was 2-30-fold over vehicle-treated mice.
Stimulation index value for MeS was 0.8 at each of the treatment
concentrations of 1, 2.5 and 5%.
Under the conditions of this assay, MeS tested in acetone, was
considered not to be a sensitizer, however the concentrations tested
were not high enough to exclude the possibility of sensitization at
higher substance concentrations.
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