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EC number: 201-152-2 | CAS number: 78-87-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October - December, 2010
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,2-dichloropropane
- EC Number:
- 201-152-2
- EC Name:
- 1,2-dichloropropane
- Cas Number:
- 78-87-5
- Molecular formula:
- C3H6Cl2
- IUPAC Name:
- 1,2-dichloropropane
- Details on test material:
- Name. 1,2-dichloropropane
Storage: room temperature
Purity: 99.59%
Description: clear liquid
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- in TA98 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA100 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA1535 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in TA1537 at 5000 and 1581 μg/plate (-S9 Mix); 5000 μg/plate (+S9 Mix)
in E. coli WP2 uvrA at 5000 μg/plate (±S9 Mix) - Vehicle / solvent:
- Dimethyl sulfoxide (DMSO) and distilled water.
Controls
- Untreated negative controls:
- yes
- Remarks:
- Dimethyl sulfoxide (DMSO); Distilled water:
- Positive controls:
- yes
- Remarks:
- 4-nitro-1,2-phenylene-diamine ; Sodium azide; 9-aminoacridine; Methyl-methanesulfonate; 2-aminoanthracene
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test item 1,2-Dichloropropane had no mutagenic activity on the growth of the applied bacterium tester strains under the test conditions used in this study.
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