Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 29 - December 02, 2010
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to
Guideline:
EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II)
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name: 1,2-dichloropropane

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
The temperature was in the range of 22.7 – 22.9 °C measured in the flask and between 21.8 and 23.1 °C measured within the climate chamber.
pH:
The range of the pH was 7.24 – 7.90 at the start and 8.12 – 8.72 at the end of the study

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 7.95 mg/L
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
7.95 mg/L
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
> 7.95 mg/L
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

The cell density in the control cultures increased by a factor of 68.17 withinthree days.

The mean coefficient of variation for section-by-section specific growth rates
(days 0-1; 1-2; 2-3) in the control cultures was 11.65 %.

The coefficient of variation of average specific growth rates during the whole test period (day 0-3) in the control cultures was 1.30 %.

All validity criteria were met, therefore the study can be considered as valid.

Applicant's summary and conclusion

Conclusions:
Under the conditions of this algal growth inhibition test, no toxic effect was observed up to the maximum achieved concentration level of 7.95 mg/L.
Correspondingly the No Observed Effect Concentration was determined as 7.95 mg/L, the 72h EC50 values were higher than 7.95 mg/L.