Tetrabromophthalic anhydride

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed prior to international guidlines and no information on GLPs. Study is well reported.

Principles of method if other than guideline:

Designed to conform to the then-most-recent draft of the Guidelines for Registering pesticides in the United States (Jan 30, 1978). Krezeminiski et al. (1975. J Agr food Chem 23:1060) and Schultz (1973. J Agr Food Chem 21:186) were used as guides.

GLP compliance:

not specified

Radiolabelling:

yes

Details on sampling:

Water sampled daily. Fish sampled on Rx days 1,3,7,10,14,21, 28 and Withdrawal days 1, 3, 7, 10 and 14.

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Stock concentration 16.91 ppm
Solvent Carrier 10% Acetone-water
Aquaria final concentration 0.01 ppm

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

Fish suplied by alex Fish Co., San Rafael, CA. Conditioned for 14 d prior to treatment. 05 - 2.0 g.

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Total depuration duration:

14 d

Reference substance (positive control):

no

Details on estimation of bioconcentration:

See above.

Type:

BCF

Value:

0

Basis:

edible fraction

Remarks on result:

other: Concentration (ppm) in tissue at all timepoints was <0.01 ppm

Type:

BCF

Value:

0

Basis:

non-edible fraction

Remarks on result:

other: Concentration in tissue at all time points was <0.01 ppm.

Results of TBPA bioconcentration study in fish.

Interval (d)	[Tetrabromophthalic anhydride; ppm]
Treatment	Water	Fish
		Edible	Viscera
1	0.010	<0.01	<0.01
3	0.010	<0.01	<0.01
7	0.008	<0.01	<0.01
10	-	<0.01	<0.01
14	0.011	<0.01	<0.01
21	0.009	<0.01	<0.01
28	0.011	<0.01	<0.01
Withdrawal
1	0.002	<0.01	<0.01
3	ND	<0.01	<0.01
7	ND	<0.01	<0.01
10	ND	<0.01	<0.01
14	ND	<0.01	<0.01

Validity criteria fulfilled:

yes

Conclusions:

TBPA was not bioaccumulated by fish (BCF=0).

Executive summary:

The mean concentration of TBPA in water, based on liquid scintillation counting of 14C-activity, during the treatment phase was 0.0098 +/- 0.001 ppm. This level was reached within 2 days after initiation. Combustion analysis of fish samples collected during the treatment and withdrawal phases indicate that the test artical was not accumulated by the fish (BCF=0).

The method of analysis would detect any 14C-activity derived from the test substance, including parent molecule, metabolites and hydrolysis products (e.g. TBPA-acid).

Results of TBPA bioconcentration study in fish.

Interval (d)	[Tetrabromophthalic anhydride; ppm]
Treatment	Water	Fish
		Edible	Viscera
1	0.010	<0.01	<0.01
3	0.010	<0.01	<0.01
7	0.008	<0.01	<0.01
10	-	<0.01	<0.01
14	0.011	<0.01	<0.01
21	0.009	<0.01	<0.01
28	0.011	<0.01	<0.01
Withdrawal
1	0.002	<0.01	<0.01
3	ND	<0.01	<0.01
7	ND	<0.01	<0.01
10	ND	<0.01	<0.01
14	ND	<0.01	<0.01

Description of key information

TBPA was not bioaccumulated by fish (BCF=0).

Key value for chemical safety assessment

BCF (aquatic species):

0 dimensionless