Registration Dossier

Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed prior to international guidlines and no information on GLPs. Study is well reported.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978

Materials and methods

Principles of method if other than guideline:
Designed to conform to the then-most-recent draft of the Guidelines for Registering pesticides in the United States (Jan 30, 1978). Krezeminiski et al. (1975. J Agr food Chem 23:1060) and Schultz (1973. J Agr Food Chem 21:186) were used as guides.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
14C-label in aromatic ring; uniformly labeled. Specific acitivity = 11.6 mCi/mmole
Diluted with non-radiolabeled material to yield a final specific activity of 2.32 mCi/mmole
Radiolabelling:
yes

Sampling and analysis

Details on sampling:
Water sampled daily. Fish sampled on Rx days 1,3,7,10,14,21, 28 and Withdrawal days 1, 3, 7, 10 and 14.

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
Stock concentration 16.91 ppm
Solvent Carrier 10% Acetone-water
Aquaria final concentration 0.01 ppm

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
Fish suplied by alex Fish Co., San Rafael, CA. Conditioned for 14 d prior to treatment. 05 - 2.0 g.

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d
Total depuration duration:
14 d

Test conditions

Reference substance (positive control):
no
Details on estimation of bioconcentration:
See above.

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
0
Basis:
edible fraction
Remarks on result:
other: Concentration (ppm) in tissue at all timepoints was <0.01 ppm
Type:
BCF
Value:
0
Basis:
non-edible fraction
Remarks on result:
other: Concentration in tissue at all time points was <0.01 ppm.

Any other information on results incl. tables

Results of TBPA bioconcentration study in fish.

Interval (d)

[Tetrabromophthalic anhydride; ppm]

Treatment

Water

Fish

Edible

Viscera

1

0.010

<0.01

<0.01

3

0.010

<0.01

<0.01

7

0.008

<0.01

<0.01

10

-

<0.01

<0.01

14

0.011

<0.01

<0.01

21

0.009

<0.01

<0.01

28

0.011

<0.01

<0.01

Withdrawal

1

0.002

<0.01

<0.01

3

ND

<0.01

<0.01

7

ND

<0.01

<0.01

10

ND

<0.01

<0.01

14

ND

<0.01

<0.01

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
TBPA was not bioaccumulated by fish (BCF=0).
Executive summary:

The mean concentration of TBPA in water, based on liquid scintillation counting of 14C-activity, during the treatment phase was 0.0098 +/- 0.001 ppm. This level was reached within 2 days after initiation. Combustion analysis of fish samples collected during the treatment and withdrawal phases indicate that the test artical was not accumulated by the fish (BCF=0).

The method of analysis would detect any 14C-activity derived from the test substance, including parent molecule, metabolites and hydrolysis products (e.g. TBPA-acid).

Results of TBPA bioconcentration study in fish.

Interval (d)

[Tetrabromophthalic anhydride; ppm]

Treatment

Water

Fish

Edible

Viscera

1

0.010

<0.01

<0.01

3

0.010

<0.01

<0.01

7

0.008

<0.01

<0.01

10

-

<0.01

<0.01

14

0.011

<0.01

<0.01

21

0.009

<0.01

<0.01

28

0.011

<0.01

<0.01

Withdrawal

1

0.002

<0.01

<0.01

3

ND

<0.01

<0.01

7

ND

<0.01

<0.01

10

ND

<0.01

<0.01

14

ND

<0.01

<0.01