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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Reported by the U.S. National Toxicology Program, performed according to good laboratory practices. Tests performed by two independent laboratories, each in triplicate.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993

Materials and methods

Principles of method if other than guideline:
See Zeiger et al. 1985. Environ Mutagen. 7:213-232.
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
Tetrachlorophthalic anhydride
EC Number:
204-171-4
EC Name:
Tetrachlorophthalic anhydride
Cas Number:
117-08-8
IUPAC Name:
4,5,6,7-tetrachloro-2-benzofuran-1,3-dione
Details on test material:
See rat and mouse 13 week studies

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
rat S9
Test concentrations with justification for top dose:
0, 33.3, 100, 333.3, 1000, 3333.3, 6666.7 ug/plate
Controls
Untreated negative controls:
yes
Positive controls:
yes
Details on test system and experimental conditions:
See Zeiger et al. (1985) Environ. Mutagen 7:213-232.

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Tetrachlorophthalic anhydride was negative in the Ames test without and without metabolic activation.
Executive summary:

Tetrachlorophthalic anhydride was negative in the Ames test without and without metabolic activation.