Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-323-0 | CAS number: 612-83-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In a study equivalent to OECD 203 (reliability category 1) with Bracydanio rerio an LC50 after 96 hours of 3.3 mg/L 3,3´-dichlorobenzidine was determined.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.3 mg/L
Additional information
For the determination of the toxicity of the submission substance 3,3´-dichlorobenzidine dihydrochloride its corresponding base, 3,3´-dichlorobenzidine, being of equivalent toxicological concern, had been tested in short-term toxicity studies in fish, aquatic invertebrates (Daphnia magna), aquatic plants (algae, Scenedesmus subspicatus) and microorganisms.
The key study for fish (reliability category 1) had been performed on Bracydanio rerio (HAMILTON - BUCHANAN) equivalent to OECD 203 fulfilling all the validity criteria of the guideline. The LC50 after 96 hours had been determined to be 3.3 mg/L 3,3´-dichlorobenzidine , although abnormal behaviour had been observed already at the lowest tested concentration of 1 mg/L . Other LC50 -values for fish species from the secondary literature (ECOTOX-database) are between 1.1 and 2.8 mg/L (all Pimephales promelas) 3,3´-dichlorobenzidine and thus not in contradiction to the key study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
