Registration Dossier
Registration Dossier
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EC number: 210-323-0 | CAS number: 612-83-9
Endpoint summary
Administrative data
Description of key information
Additional information
For the determination of the toxicity of the submission substance 3,3´-dichlorobenzidine dihydrochloride its corresponding base, 3,3´-dichlorobenzidine, being of equivalent toxicological concern, had been tested in short-term toxicity studies in fish, aquatic invertebrates (Daphnia magna), aquatic plants (algae, Scenedesmus subspicatus) and microorganisms.
The key study for fish (reliability category 1) had been performed on Brachydanio rerio (HAMILTON - BUCHANAN) equivalent to OECD 203 fulfilling all the validity criteria of the guideline. The LC50 after 96 hours had been determined to be 3.3 mg/L 3,3´-dichlorobenzidine.
Other LC50 -values for fish species from the secondary literature (ECOTOX-database) are between 1.1 and 2.8 mg/L (all Pimephales promelas) 3,3´-dichlorobenzidine and thus not in contradiction to the key study.
The key study for aquatic invertebrates (reliability category of 1) had been performed on Daphnia magna STRAUS equivalent to OECD 202, fulfilling all the validity criteria of the guideline. The EC50 (immobilization) after 48 hours had been determined to be 2.47 mg/L 3,3´-dichlorobenzidine with a 95% confidence interval of 2.12 - 2.86 mg/L. Another EC50 -value (mortality) for daphnia magna from secondary source (ECOTOX-database) is 1.05 mg/L 3,3´-dichlorobenzidine and thus well in accordance with the result of the key study. The EC0 after 48 h as reported in the key study was 1.14 mg/L.
The key study for aquatic plants (reliability category 1) had been performed on the unicellular alga Scenedesmus subspicatus. In a growth inhibition test according to OECD 201 fulfilling all the validity criteria demanded by OECD 201 as well as the requirements on the reporting on experimental procedures and results, an EC50 (growth reduction) after 72 hours of 4.3 mg/L 3,3´-dichlorobenzidine with a 95% confidence interval of 3.2 - 5.6 mg/L in comparison to the solvent control group had been determined. The no observed effect concentration (NOEC) as defined in the OECD-test guidelines (no significant growth inhibition and no cell deformation) was found after 72 hours to be 0.32 mg/L 3,3´-dichlorobenzidine.
Microorganisms: With the exception of Photobacterium phosphoreum (EC50 light emission = 0.058 mg/L, no incubation duration given), the secondary literature reports effect values well above the solubility limit of 3,3´-dichlorobenzidine in water (3 mg/L) or no effect at all for activated sludge bacteria and several single strains.
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