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Terrestrial studieshave been conducted with soil micoroganaims in a nitrogen transformation assay, earthworms, and plants.

A nitrogen transformation test was performed according to GLP and OECD Guideline 216. Following a preliminary range-finding test soil microorganisms were exposed to the test material at a single concentration of 1000 mg/kg for 28 days. DMH showed no significant effect on the nitrogen transformation activity of soil microorganisms at a test concentration of 1000 mg/kg over a 28-Day period.

The acute toxicity study in earthworms was conducted according to OECD Guideline 207 and gave a 14-day LC50 value greater than 1000 mg/kg.

Toxicity to terrestrial plants (Soybean, cucumber and oat) was investigated in a seedling and emergence study conducted to GLP and OECD Guideline 208. DMH was tested at nominal concentrations of 40, 100, 160, 400 and 1000 mg/kg. The lowest EC50 derived was for cucumber which was 990 mg/kg.