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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test substance is considered not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

In a ready biodegradability test, performed according to OECD guideline 301A, biodegradation of the testsubstance by micro-organisms was determined (CG, 1987).

The test substance (40 mg/L) was exposed to a mixture of polyvalent bacteria from an effluent of a domestic sewage treatment plant (ARA-Rhein) for 28 days. After 28 days no biodegradation was observed. Biological oxygen demand in 5 days (BOD5) was determined in a study performed according to the EEC, Annex V, part C.8 guideline (CG 1987). The testsubstance (1, 5, 10, 20, 50, 100, 200 and 500 mg/L) was exposed to a mixed culture of bacteria (effluent of a Husman laboratory apparatus). The BOD5 of the testsubstance, corrected by the blank control was 0 mg/g oxygen.

The chemical oxygen demand for the testsubstance was determined in a test following the EEC Directive 79-831, Annex V, C.9.

according to German DIN-Norm 38'409, H-41, 1 (Dezember 1980). The test was carried out correctly and the results are valid. The conclusion is that the COD for the test substance is 820 mg/g O2. The BOD5 and COD results support the conclusion that the test substance is not readily biodegradable according to the presented data.