Trisodium 7-(4-(4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazine- 2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 February 1987 to 06 March 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

yes

Remarks:

Sterilisation instead of sterrl filtration - Determination of bacteria concentration: by plating

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
Batch No.: Op. 1/86, Vers. 10/86

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Details on inoculum:

Mixture of polyvalent bacteria collected on 06.02.87
- effluent of a domestic sewage treatment plant (ARA-Rhein)
Bacteria concentration: 1,4E+06/mL

Duration of test (contact time):

28 d

Initial conc.:

40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

- Test concentration: 40 mg/L DOC nominal
- Water / Nutrient: According to the specification of the EEC Annex V, Part. C.4 resp. OECD Method 301A.
- Temperature: 20 - 23 °C
- Duration: 28 days
- Lightening: Indirect daylight
- Estimation technique: TOC/DOC SHIMADZU TOC-500

Reference substance:

other: glucose

Preliminary study:

no data

Test performance:

Biodegradation corrected by the blank control and measured as DOC (mg/1) is: 0 % (14th day)

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

14 d

Remarks on result:

other: No further details provided

Details on results:

In the result of this degradation test of FAT 40224/C only DOC values until day 14 were considered for the final calculation. The DOC values of day 28 were not taken into consideration. The measured DOC value in the inoculum control, flask 7, on day 28 is inexplicably high. Due to this fact the calculated degradation value on day 28 of the test substance can not be regarded as a real degradation rate. It has to be kept in mind, however, that the gross DOC values of flasks 8, 9 and 10 were all about the same on day 7, 14 and 28 therefore a degradation of FAT 40224/C does not seem to be very likely.

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/L) is: 100 % (14th day)

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

FAT 40224/C is not readily biodegradable under the conditions of this test.

Executive summary:

The biodegradability was determined as percent of DOC (Dissolved Organic Carbon) for the substance according to Guideline EEC Directive, Annex V, C.4 according to OECD Guideline for testing chemicals No.301A, Paris 1981. The results indicate that biodegradation (14d) of the test substance is 0 %. Based on the study results the test substance is not readily biodegradable under the conditions of this test.

Description of key information

The ready biodegradability was determined as percent of DOC (Dissolved Organic Carbon) for the substance according to Guideline EEC Directive, Annex V, C.4 according to OECD Guideline for testing chemicals No.301A, Paris 1981. The results indicate that Biodegradation (14d) of the test substance is 0 %. Based on the study results the test substance is not readily biodegradable under the conditions of this test. 

The biochemical oxygen demand for the test substance was determined in a test following the EEC Directive 79-831 Annex V, Part. C.8, according to DEV H-5 (a2) Deutsche Einheitsverfahren, 1966. The test was carried out correctly and the results are valid. The conclusion is that the BOD5 for the test substance is 0 mg/g O2. The chemical oxygen demand for the test substance was determined in a test following the EEC Directive 79-831, Annex V, C.9. according to German DIN-Norm 38'409, H-41, 1 (Dezember 1980). The test was carried out correctly and the results are valid. The conclusion is that the COD for the test substance is 820 mg/g O2. The BOD5 and COD results support the conclusion that the test substance is not readily biodegradable according to the presented data. In addition, Reactive Yellow 174 was reported to be not readily biodegradable in a 2019 study conducted by MITI, Japan. The details about the study are not available.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater