Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 218-747-8 | CAS number: 2224-33-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Test method similar to OECD Guideline 476. No data on GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- not specified
- Type of assay:
- other: mammalian cell gene mutation assay
Test material
- Reference substance name:
- Butanone oxime
- EC Number:
- 202-496-6
- EC Name:
- Butanone oxime
- Cas Number:
- 96-29-7
- Molecular formula:
- C4H9NO
- IUPAC Name:
- butan-2-one oxime
- Details on test material:
- - Name of test material (as cited in study report): 2-Butanone oxime
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix (prepared from Aroclor 1254-induced male Fisher 344 rats)
- Test concentrations with justification for top dose:
- Without metabolic activation: 0 (control), 2.8, 3.6, 4.6, 5.5 and 6.5 µl/mL
With metabolic activation: 0 (control), 1.7, 2.8, 3.6, 4.6, 5.5 and 6.5 µl/mL - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Dimethyl sulfoxide
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Positive controls:
- yes
- Positive control substance:
- other: 3-methylcholanthrene
Results and discussion
Test results
- Key result
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- (only with cytotoxicity in the absence of S9)
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- 2-butanone oxime showed evidence of mutagenic activity in the absence of S9 activation, but in the presence of cytotoxicity (it was noted that there was a dose response for both mutant frequency and relative total colony growth). No evidence of mutagenic activity was observed in the presence of S9 activation.
Any other information on results incl. tables
Results obtainend without metabolic activation:
Dose (µg/mL) |
Relative total growth (% of control) |
Mutant frequency (100,000 survivors) |
0 |
100 |
0.50 |
2.8 |
50.0 |
0.75 |
3.6 |
35.5 |
0.95 |
4.6 |
28.5 |
1.3 |
5.5 |
12.5 |
2.0 |
6.5 |
7.5 |
2.65 |
There was a dose response for both mutant frequency and relative total colony growth.
Applicant's summary and conclusion
- Conclusions:
- 2-butanone oxime was negative for mutagenic activity in mouse lymphoma L5178Y cells in the presence of S9 metabolic activation, but was positive in the absence of S9 metabolic activation and in the presence of cytotoxicity.
- Executive summary:
A mammalian cell mutagenicity test was performed on 2 -butanone oxime in accordance with a test method similar to OECD Guideline 476. Mouse lymphoma L5178Y cells were exposed to concentrations up to 6.5 µg/plate with and without metabolic activation S9 mix prepared from Aroclor 1254-induced male Fisher 344 rats. Negative and positive controls were performed satisfactorily. 2-butanone oxime was negative for mutagenic activity in mouse lymphoma L5178Y cells in the presence of S9 metabolic activation, but was positive in the absence of S9 metabolic activation and in the presence of cytotoxicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
