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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 21, 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
The method used was equivalent to OECD Guideline 203.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 234, 390, 650, 1084 and 1806 mg/L.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Yes (negative control, without test item)
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead Minnow
- Age at study initiation (mean and range, SD): 30 days
- Length at study initiation (length definition, mean, range and SD): 21.2 ± 2.296 mm
- Weight at study initiation (mean and range, SD): 0.148 ± 0.0496 g
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
44.9 CaCO3 mg/L
Test temperature:
24.4 ± 0.91 ºC
pH:
7.77 ± 0.04
Dissolved oxygen:
6.8 ± 0.48 mg/L
Nominal and measured concentrations:
Nominal concentration: 0 (control), 234, 390, 650, 1084, 1806 mg/L.
Measured concentrations: <10, 215.5, 394.5, 580.5, 955, 1730.5 mg/L (mean values).
Percent recovery: 106.9±2.8%
Details on test conditions:
TEST SYSTEM
- Test vessel: Tanks
- Material, size, headspace, fill volume: 6.3 L
- No. of organisms per vessel: 25
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.587 g/L

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 38.0 mg/L CaCO3

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities and clinical effects of each replicate were observed at 1, 2, 3, 4, 6, 12, 24, 48, 72 and 96 hours of exposure.
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
843 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (conf. lim: 777-914 mg/L)
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
843 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: (conf. lim.: 777-914 mg/L)
Details on results:
- Behavioural abnormalities: Affected fish stopped schooling and lost equilibrium prior to death.

Validity criteria fulfilled:
yes
Remarks:
(No mortality was observed by the end of the test control, the substance concentration was maintained during the test within 80%)
Conclusions:
The 96-LC50 in Fathead Minnows fish for test item 2-butanone oxime was determined to be 843 mg/L, for both based on mortality and based on behavioural effects.
Executive summary:

An acute toxicity test with test item 2 -butanone oxime on Fathead Minnows (Pimephales promelas) was performed in accordance with an equivalent method to OECD Guideline 203. 25 fish for tank and replicate (2 replicates per dose) were exposed for 96 hours to nominal 2 -butanone oxime concentrations of 0 (control), 234, 390, 650, 1084 and 1806 mg/L. Analytical monitorization was performed during test performance. The mortality and effect of fish were observed at 1, 2, 3, 4, 6, 12, 24, 48, 72 and 96 hours of exposure. The 96h-LC50 in Fathead Minnows fish for test item 2 -butanone oxime was determined to be 843 mg/L, both based on mortality and based on behavioural effects.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Studies conducted by the Japanese Ministry of the Environment based on OECD-GLP standard and OECD test guidelines.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Remarks:
(no details on method are given)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Measurement frequency: start of exposure, before renewal of test solution
Test organisms (species):
Oryzias latipes
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
24 ± 1 ºC
Nominal and measured concentrations:
0 (control), 100 mg/L (nominal)
No data on measured concentrations.
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: 2.5 L
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): 48 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark
- Light intensity: room light

Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Validity criteria fulfilled:
not specified
Conclusions:
The 96h-LC50 for 2-butanone oxime in fish (Oryzias lapides) was determined to be >100 mg/L.
Executive summary:

A fish acute toxicity test was peformed by the Japanese Ministry of the Environment according to OECD TG 203. Oryzias lapides (5 for replicate) were exposed to 0 (control) and 100 mg/L 2 -butanone oxime under-semi static conditions for 96 hours. The 96h-LC50 for test substance 2 -butanone oxime in fish (Oryzias lapides) was determined to be >100 mg/L.

Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
Butan-2-one O,O',O''-(vinylsilanetriyl)oxime undergoes rapid hydrolysis in aqueous to butanone oxime and the corresponding silanol. Silanetriol undergo continuous condensation reactions to produce higher molecular weight siloxanes which are considered biologically unavailable. Therefore, the observed toxicity is likely due to butanone oxime and their values are comparable.
See attached the reporting format.
Reason / purpose:
read-across source
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 011.11 mg/L
Nominal / measured:
estimated
Conc. based on:
other: (analogue substance)
Basis for effect:
mortality
Remarks on result:
other: (Based on read-across approach from an analogue)
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1 011.11 mg/L
Nominal / measured:
estimated
Conc. based on:
other: (analogue substance)
Basis for effect:
behaviour
Remarks on result:
other: (based on read-across approach from an analogue)
Details on results:
Based on the experimental results obtained with the analogue substance butanone oxime (96h LC50 =843 mg/L ), the read-across approach is applied and the 96h LC50 for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was calculated to be 1011.11 mg/L.
Validity criteria fulfilled:
not applicable
Conclusions:
Based on the read-across approach from experimental results on analogue butanone oxime, the 96-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 1011.11 mg/L based on mortality.
Executive summary:

An acute toxicity test on Fathead Minnows (Pimephales promelas) was performed with the analogue substance butanone oxime in accordance with a method equivalent to OECD Guideline 203. 25 fish for tank and replicate (2 replicates per dose) were exposed for 96 hours to concentrations up to 1806 mg/L and the 96h-LC50 in fish was determined to be 843 mg/L (basis for effect: mortality). Based on these results, the read-across approach was performed and the 96-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 1011.11 mg/L.

Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
Butan-2-one O,O',O''-(vinylsilanetriyl)oxime undergoes rapid hydrolysis in aqueous to butanone oxime and the corresponding silanol. Silanetriol undergo continuous condensation reactions to produce higher molecular weight siloxanes which are considered biologically unavailable. Therefore, the observed toxicity is likely due to butanone oxime and their values are comparable.
See attached the reporting format.
Reason / purpose:
read-across source
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 119.94 mg/L
Nominal / measured:
estimated
Conc. based on:
other: (analogue substance)
Basis for effect:
mortality
Remarks on result:
other: (Based on read-across approach from an analogue)
Details on results:
Based on the experimental results obtained with the analogue substance butanone oxime (96h LC50 >100 mg/L ), the read-across approach is applied and the 96h LC50 for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was calculated to be 119.94 mg/L.
Validity criteria fulfilled:
not applicable
Conclusions:
Based on the read-across approach from experimental results on analogue butanone oxime, the 96h-LC50 for butan-2-one O,O',O''-(vinylsilanetriyl)oxime in fish was determined to be >119.94 mg/L.
Executive summary:

A fish acute toxicity test was performed on analogue substance butanone oxime by the Japanese Ministry of the Environment according to OECD TG 203. The 96h-LC50 in fish (Oryzias lapides) was determined to be >100 mg/L. Based on these results, the read-across approach was performed and the 96-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be > 119.94 mg/L.

Description of key information

Key study: Read-across from experimental data on analogue butanone oxime: The 96-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 1011.11 mg/L.

Key study: Read-across from experimental data on analogue butanone oxime: The 96h-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be >119.94 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
1 011.11 mg/L

Additional information

Read-across approach from analogue butanone oxime:

Key study: Based on the read-across approach from experimental data on analogue butanone oxime (test method equivalent to OECD 203, 96h-LC50 in Fathead Minnows (Pimephales promelas) = 843 mg/L based on mortality), the 96-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 1011.11 mg/L.

Key study: Based on the read-across approach from experimental data on analogue butanone oxime (test method according to OECD TG 203, 96h-LC50 in Oryzias lapides fish >100 mg/L), the 96h-LC50 in fish for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be >119.94 mg/L.