Butan-2-one O,O',O''-(vinylsilylidyne)trioxime

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Studies conducted by the Japanese Ministry of the Environment based on based on OECD-GLP standard and OECD test guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

Remarks:

(no details on test performance are given)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Measurement frequency: start of exposure, before renewal of test solution

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Strain: ATCC 22662

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

23 ± 2 ºC

Nominal and measured concentrations:

Nominal concentration: 0 (control), 1.02, 2.56, 6.40, 16.0, 40.0 mg/L.
Measurement concentrations: not available.

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 100 mL
- Shaking: 100 rpm.
- Initial cells density: 10E4 cells.
- No. of vessels per concentration (replicates): 3

OTHER TEST CONDITIONS
- Light intensity and quality: 4000-5000 lux

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.5

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

2.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

6.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: area under growth curve

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: area under growth curve

Validity criteria fulfilled:

not specified

Conclusions:

The 72h-EC50 and 72h-NOEC based on algae (Selenastrum capricorntum) growth rate for 2-butanone oxime was determined to be 16 mg/L and 2.6 mg/L respectively.

Executive summary:

An Algal Growth Inhibition Test was performed by the Japanese Ministry of the Environment in accordance with OECD TG 201. Selenastrum capricornutum were exposed to 0 (control), 1.02, 2.56, 6.40, 16.0, 40.0 mg/L of 2 -butanone oxime for 72h under static conditions. The 72h-EC50 and 72h-NOEC based on algae (Selenastrum capricornutum) growht rate for test substance 2 -butanone oxime was determined to be 16 mg/L and 2.6 mg/L respectively. The 72 -EC50 and 72h-NOEC based on the area under growth curve (AUG) for 2 -butanone oxime was determined to be 6.1 mg/L and 1.0 mg/L.

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH
Butan-2-one O,O',O''-(vinylsilanetriyl)oxime undergoes rapid hydrolysis in aqueous to butanone oxime and the corresponding silanol. Silanetriol undergo continuous condensation reactions to produce higher molecular weight siloxanes which are considered biologically unavailable. Therefore, the observed toxicity is likely due to butanone oxime and their values are comparable.
See attached the reporting format.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

19.19 mg/L

Nominal / measured:

estimated

Conc. based on:

other: (analogue substance)

Basis for effect:

growth rate

Remarks on result:

other: (Based on read-across approach from an analogue)

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

3.12 mg/L

Nominal / measured:

estimated

Conc. based on:

other: (analogue substance)

Basis for effect:

growth rate

Remarks on result:

other: (Based on read-across approach from an analogue)

Details on results:

Based on the experimental results obtained with the analogue substance butanone oxime (72h EC50 =16 mg/L, NOEC = 2.60 mg/L ), the read-across approach is applied and the 72h EC50 and NOEC for butan-2-one O,O',O''-(vinylsilanetriyl)oxime were calculated to be 19.19 mg/L and 3.12 mg/L respectively.

Validity criteria fulfilled:

not applicable

Conclusions:

Based on read-across approach from experimental results on analogue butanone oxime, the 72h-EC50 and 72h-NOEC based on algae growth rate for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 19.19 mg/L and 3.12 mg/L respectively.

Executive summary:

An Algal Growth Inhibition Test was performed on analogue substance butanone oxime by the Japanese Ministry of the Environment in accordance with OECD TG 201. The 72h-EC50 and 72h-NOEC based on algae (Selenastrum capricornutum) growth rate was determined to be 16 mg/L and 2.6 mg/L respectively. Based on these results the read-across approach was performed and the 72h -EC50 and 72h-NOEC based on algae growth rate for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 19.19 mg/L and 3.12 mg/L respectively.

Description of key information

Key study: Read-across approach from experimental results on analogue butanone oxime: The 72h-EC50 and 72h-NOEC based on algae growth rate for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 19.19 mg/L and 3.12 mg/L respectively.

Key value for chemical safety assessment

EC50 for freshwater algae:

19.19 mg/L

EC10 or NOEC for freshwater algae:

3.12 mg/L

Additional information

Key study: Read-across approach from analogue butanone oxime:

Based on the read-across approach from experimental results on analogue butanone oxime (test method OECD 201, 72h-EC50 = 16 mg/L and 72h-NOEC = 2.6, based on algae growth rate), the algae 72h -EC50 and 72h-NOEC for butan-2-one O,O',O''-(vinylsilanetriyl)oxime was determined to be 19.19 mg/L and 3.12 mg/L (basis for effect: growth rate) respectively.