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EC number: 446-990-1
CAS number: -
A study was performed to assess the acute toxicity of the test material
to Daphnia magna. The method followed that described in the OECD
guidelines for the testing of chemicals n°202, "Daphnia sp, acute
immobilisation test" referenced as method C.2 of Comission Directive
Following a preliminary range-finding test, twenty daphnids (4
replicates of 5 animals) were exposed to an aqueous solution of the test
material at a concentration of 100 mg/L for 48 hours at a temperature of
20.8 to 21.3 °C under static conditions. Immobilisation and any adverse
reactions to exposure were recorded after3, 24 and 48 hours.
The 48 -hour EC50 for the test material to Daphnia magna based on
nominal test concentrations was greater than 100 mg/L and
correspondingly the No Observed Effect Concentration was 100 mg/L.
Analysis of the test preparations at 0 and 48 hours showed measured test
concentrations to range from 99% to 108% of nominal value and so results
are based on nominal test concentrations only.
It was considered unnecessary and unrealistic to test at concentrations
in excess of 100 mg/L.
The 48h-EC50 for the reference material to Daphnia magna based on
nominal concentrations was 1.1 mg/L with 95% confidence limits of 0.97 -
1.2 mh/L. The No Observed Effect Concentration was 0.56 mg/L.
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