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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study, OCDE guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Directive CE - méthode C2 et OCDE 202.
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
2003-02-13

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: compact
Details on test material:
Sponsor's identification
Description: yellow solid block
Batch number: 173 JG1
Date received: 29 april 2005
Storage conditions: room temperature in the dark

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna derived from in-house laboratory cultures.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
CA 250 mg CaCO3/L
Test temperature:
from 20.8 to 21.3 °C
Dissolved oxygen:
8.2 to 8.3 mg O2/L
Nominal and measured concentrations:
nominal concentration: 0 and 100 mg/L
measured concentration:
Details on test conditions:
200 mL glass jars
5 daphnids in each vessel
four replicates test and control vessels were prepared
Reference substance (positive control):
yes
Remarks:
K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Results with reference substance (positive control):
48h-EC50 was 1.1 mg/L
NOEC= 0.56 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
48h-EC50> 100 mg/L
NOEC= 100 mg/L
Executive summary:

A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD guidelines for the testing of chemicals n°202, "Daphnia sp, acute immobilisation test" referenced as method C.2 of Comission Directive 92/69/EEC.

Methods

Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 animals) were exposed to an aqueous solution of the test material at a concentration of 100 mg/L for 48 hours at a temperature of 20.8 to 21.3 °C under static conditions. Immobilisation and any adverse reactions to exposure were recorded after3, 24 and 48 hours.

Results

The 48 -hour EC50 for the test material to Daphnia magna based on nominal test concentrations was greater than 100 mg/L and correspondingly the No Observed Effect Concentration was 100 mg/L.

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 99% to 108% of nominal value and so results are based on nominal test concentrations only.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

The 48h-EC50 for the reference material to Daphnia magna based on nominal concentrations was 1.1 mg/L with 95% confidence limits of 0.97 - 1.2 mh/L. The No Observed Effect Concentration was 0.56 mg/L.