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EC number: 446-990-1
CAS number: -
Adult Mortality and Treatment Mean Bodyweights (mg) are displayed in the
(mg/kg dry soil)
Day of study
5 mg a.i./kg dry soil
- not applicable
are for comparison with water control using Williams’ test for the
treatment rates and t-test for the Mascot Systemic
Number of Juvenile Worms per Treatment Replicate are displayed in the
All groups were compared against the
water control group using Dunnett’s test
A preliminary range finding study was conducted at 0.1, 1, 10, 100
and 1000 mg/kg dry soil to determine the acute toxicity of
D-glucopyranose, oligomers, xylityl glycosides and 1,4 anhydro D-xylitol
and D-xylitolto the earthworm Eisenia fetida and to ensure
selection of appropriate treatment rates for the definitive reproduction
A study was then performed to determine the effects of D-glucopyranose,
oligomers, xylityl glycosides and 1,4 anhydro D-xylitol and D-xylitolon
the reproduction and growth of the earthworm, Eisenia fetida, in
an artificial soil under laboratory conditions. The
method followed was that described in OECD 222 Guideline for the testing
of Chemicals, Earthworm Reproduction Test (Eisenia fetida/Eisenia
andrei) 29 July 2016.
The test substance name D-glucopyranose, oligomers, xylityl
glycosides and 1,4 anhydro D-xylitol and D-xylitol will be referred to
as D-glucopyranose throughout the remaining report.
Ten groups of worms were allocated to the reproduction study. Eight
groups of 40 worms were treated with D-glucopyranose at test rates of
16.3, 29.4, 52.9, 95.3, 171.5, 308.6, 555.6 and 1000 mg/kg dry soil
weight, mixed into the soil. A negative control group
of 80 worms, was maintained in untreated soil, and a positive control
group of 80 worms, was treated with Mascot Systemic (active ingredient
carbendazim) at 5 mg a.i./kg dry soil weight. Adult
worms were removed from the soil four weeks after treatment and the
juvenile worms reared for a further four weeks.
The preliminary range finding study indicated that the LC50value
was >1000 mg/kg dry soil. No mortality was observed in the water control
or at any treatment rate at seven or 14 days after treatment. There were
slight reductions in bodyweight of 1.3 and 1% at 1 and 10 mg/kg dry soil
respectively in comparison to the water control group. All worms
In the reproduction study no mortality was observed in the water control
or at any D-glucopyranose treatment rate. Mortality of
1.25% was recorded in the toxic reference treatment at 28 days.
The LC50for adult mortality at Day 28 was >1000 mg/kg
dry soil. The confidence intervals could not be obtained. A NOEC of 1000
mg/kg dry soil was achieved.
There was no statistically significant reduction in the mean
bodyweights 28 days after treatment at any D-glucopyranose rate compared
to the water control. A significantly lower body weight (p<0.001***)
was recorded in the toxic reference group in comparison to the water
The adjusted mean bodyweights on Day 28 at rates of 16.3, 29.4,
52.9, 95.3, 171.5, 308.6, 555.6 and 1000 mg/kg dry soil were 517, 489,
499, 507, 509, 510, 551 and 522 mg respectively compared to 493 mg in
the water control and 345 mg in the toxic reference.
The LC50for mean adult bodyweight was >1000 mg/kg dry
soil. The confidence intervals could not be obtained. A NOEC of 1000
mg/kg dry soil was achieved.
There was no statistically significant reduction in the number of
juveniles produced at any treatment rate compared against the water
The mean number of juveniles produced at rates of 16.3, 29.4,
52.9, 95.3, 171.5, 308.6, 555.6 and 1000 mg/kg dry soil was 253, 232,
221, 242, 234, 227, 230 and 256 respectively compared to 234 in the
water control and 0.25 for the toxic reference.
The EC50for the number of juveniles on Day 56 was >1000 mg/kg
dry soil. A NOEC of 1000 mg/kg dry soil was achieved
compared against the water control.
The study was considered valid as there was≤10% adult mortality at four
weeks and ≥30 juveniles had been produced in each water control
replicate by the end of the test with the coefficient of variation of
reproduction ≤ 30%. In addition application of
the toxic reference Mascot Systemic at 5 mg a.i./kg dry soil resulted in
substantial and unequivocal toxic effects.
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