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EC number: 446-990-1
CAS number: -
A study was performed to assess the effect of the test material on the
growth of the green alga Scenedesmus subspicatus. The method followed
that described in the OECD guidelines for testing of chemicals n°201
"alga growth inhibition test" referenced as method C.3 of comission
Following a preliminary range-finding test, Scenedesmus subspicatus was
exposed to an aqueous solution of the test material at a concentration
of 100 mg/L (six replicate flasks) for 72 hours, under constant
illumination and shaking at a temperature of 24 +/- 1 °C.
Samples of the algal populations were removed daily and cell
concentrations determined for each control and treatment group, using a
Coulter Multisizer Particle Counter.
Exposure of Scenedesmus subspicatus to the test material gave EC50
values greater than 100 mg/L and correpondingly the No Observed Effect
Concentration was 100 mg/L.
It was considered unnecessary and unrealistic to test at concentrations
in excess of 100 mg/L.
Analysis of the test preparations at 0 hours showed measured test
concentrations to range from 107 to 108 % of nominal. Analysis of the
test preparations at 72 hours. showed a marked decline in measured test
concentrations in the range of 54 to 55 % of nominal.
Given that the preliminary stability analyses indicates that the test
material was stable in culture medium over the test duration this
decline was considered to be due to adsorption and/or absorption to
algal cells. The preliminary recovery analyses indicated that no
immediate adsorption/absorption to algal cells occured, this does
however preclude long term adsorption/absorption over the test period.
Given this decline in measured test concentrations it was considered
justifiable to base the results on the geometric mean measured
concentrations in order to give a "worst case" analyses of the data. The
EC50 values based on the geometric mean measured test concentrations
were greater than 77 mg/L and correpondingly the No Observed Effect
Concentrations was 77 mg/L.
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