Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 446-990-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline, GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2005-12-16
Test material
- Reference substance name:
- -
- EC Number:
- 446-990-1
- EC Name:
- -
- Molecular formula:
- C5H11O5 [C6H10O5]1.45 and C5H10O4 and C5H12O5
- IUPAC Name:
- 2-(hydroxymethyl)oxolane-3,4-diol; 5-{[2,3,4,5-tetrahydroxy-6-(xenoniooxy)hexyl]oxy}pentane-1,2,3,4-tetrol; pentane-1,2,3,4,5-pentol
- Details on test material:
- batch number: 173 JG-1
description: gel (at receipt), yellow hard wax (analytical certificate)
received: 2006-12-12
storage: at room temperature
purity 99.7%
hydrosolubility: 674 g/L (20°C)
expiry date: 2008-01-03
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- concentration: 100 mg/L
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test system
Groups of test and reference suspensions and controls were set up as follows:
• two control replicates containing inoculum at 1.6 g/L,
• five test concentrations containing the test item AQUAXYL at 10, 31.6, 100, 316 or
1000 mg/L and inoculum at 1.6 g/L, with one replicate per concentration,
• three reference concentrations containing the reference item 3,5-dichlorophenol at 4, 12 or
36 mg/L and inoculum at 1.6 g/L, with one replicate per concentration.
The two control replicates were used to determine the background respiration rate of the sludge
and were not exposed to the test or reference items.
Test and reference suspensions and controls were prepared in test flasks and then immediately
aerated for 3 hours before being transferred to an oxygen measuring apparatus.
Measurement of oxygen concentration of the above suspensions was determined using an
oxygen probe placed into an opaque BOD (Biological Oxygen Demand) flask filled with the test
or reference suspension which was agitated with a magnetic stirrer. The oxygen probe was
connected to a meter equipped with a chart recorder.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Type: activated sludge collected from a water treatment works containing
effluent from a predominantly domestic origin.
Origin: the water treatment plant of Evreux (France).
Reason for this choice: activated sludge is recommended in OECD guideline for this test.
Preparation: After sampling, the inoculum was left to settle and the main part of the supernatant was rejected. It was washed once with dechlorinated
water and then filtered (to remove the biggest particles).
Four samples of 50 mL were taken to determine the content in suspended matter (dry weight/v). The concentration of sludge (5.46 g/L) was then adjusted to 4 g/L by addition of the appropriate volume of dechlorinated water.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 280 +/- 20 mg/L
- Test temperature:
- between 21 and 22°C
- pH:
- 7.68
- Details on test conditions:
- Temperature: between 21°C and 22°C.
Illumination: no special precautions were taken.
Dilution water: dechlorinated tap water was used, with a hardness of 280 ± 20 mg/L
as CaCO3.
Duration of test: 3 hours.
Aeration: air was bubbled through each suspension at the rate of 0.5 to 1 L/min
until the start of oxygen concentration measurements. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- The highest test item concentration without significant effect (difference ≤ 15%) on the respiration rate, relative to mean of the controls, was ≥ 1000 mg/L.
- Results with reference substance (positive control):
- As the validity criteria were met (the difference between the respiration rates of the two controls was below 15% and the EC50 of 3,5-dichlorophenol was 13.6 mg/L i.e. between 5 and 30 mg/L), the test was considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under our experimental conditions, the 3-hour EC50 of the test item for activated sludge respiration inhibition was > 1000 mg/L.
The highest test item concentration without significant effect on the respiration was ≥ 1000 mg/L. - Executive summary:
The effect of the test item on the respiration of activated sewage sludge was evaluated using a 3-hour static test according to OECD guideline No. 209 (4th April 1984).
All concentrations in dechlorinated water experienced in this test were lower than the water solubility of this test item.
The inhibition potential of the test item on the respiration rate of an activated sludge at 1.6 g/L in dechlorinated water was investigated using the following suspensions:
• two control suspensions (activated sludge alone),
• five test item suspensions: activated sludge and test item at concentration of 10, 31.6, 100,
316 or 1000 mg/L,
• three reference item (3,5-dichlorophenol) suspensions: activated sludge and the reference item
at concentration of 4, 12 or 36 mg/L.
The five test item concentrations were prepared by dilution of a stock solution at 2000 mg/L (pH: 7.68).
The oxygen consumption (i.e. respiration rate) of the test item, reference item and control suspensions was measured for approximately 10 minutes, with a chart recorder, after an aeration of 3 hours.
The EC50 (concentration expected to cause 50% inhibition of the respiration rate) of the test item and that of the reference item were determined considering the mean oxygen consumption of the controls as 100%.
As the validity criteria were met (the difference between the respiration rates of the two controls was below 15% and the EC50 of 3,5-dichlorophenol was 13.6 mg/L i.e. between 5 and 30 mg/L), the test was considered valid.
The respiration rate of the test item suspension at the highest concentration (1000 mg/L) was equivalent to the respiration rate of the first control (i.e. these rates were within 15% of each other). Therefore, the oxygen consumption rate of the four other test item suspensions (10 to 316 mg/L) was not determined.
The highest test item concentration without significant effect (difference ≤ 15%) on the respiration rate, relative to mean of the controls, was ≥ 1000 mg/L.
Under our experimental conditions, the 3-hour EC50 of the test item for activated sludge respiration inhibition was > 1000 mg/L.
The highest test item concentration without significant effect on the respiration was ≥ 1000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
За да гарантираме, че се възползвате максимално от функциите на нашия уебсайт, сме въвели „бисквитки“.