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EC number: 446-990-1
CAS number: -
The effect of the test item on the respiration of activated sewage
sludge was evaluated using a 3-hour static test according to OECD
guideline No. 209 (4th April 1984).
All concentrations in dechlorinated water experienced in this test were
lower than the water solubility of this test item.
The inhibition potential of the test item on the respiration rate of an
activated sludge at 1.6 g/L in dechlorinated water was investigated
using the following suspensions:
• two control suspensions (activated sludge alone),
• five test item suspensions: activated sludge and test item at
concentration of 10, 31.6, 100,
316 or 1000 mg/L,
• three reference item (3,5-dichlorophenol) suspensions: activated
sludge and the reference item
at concentration of 4, 12 or 36 mg/L.
The five test item concentrations were prepared by dilution of a stock
solution at 2000 mg/L (pH: 7.68).
The oxygen consumption (i.e. respiration rate) of the test item,
reference item and control suspensions was measured for approximately 10
minutes, with a chart recorder, after an aeration of 3 hours.
The EC50 (concentration expected to cause 50% inhibition of the
respiration rate) of the test item and that of the reference item were
determined considering the mean oxygen consumption of the controls as
As the validity criteria were met (the difference between the
respiration rates of the two controls was below 15% and the EC50 of
3,5-dichlorophenol was 13.6 mg/L i.e. between 5 and 30 mg/L), the test
was considered valid.
The respiration rate of the test item suspension at the highest
concentration (1000 mg/L) was equivalent to the respiration rate of the
first control (i.e. these rates were within 15% of each other).
Therefore, the oxygen consumption rate of the four other test item
suspensions (10 to 316 mg/L) was not determined.
The highest test item concentration without significant effect
(difference ≤ 15%) on the respiration rate, relative to mean of the
controls, was ≥ 1000 mg/L.
Under our experimental conditions, the 3-hour EC50 of the test item for
activated sludge respiration inhibition was > 1000 mg/L.
The highest test item concentration without significant effect on the
respiration was ≥ 1000 mg/L.
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