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EC number: 446-990-1
CAS number: -
A study was performed to assess the acute toxicity of the test material
to rainbow trout (Oncorhynchus mykiss). The method followed that
described in the OECD guidelines for testing of chemicals (1992) n° 203,
"fish acute toxicity test" referenced as method C.1 of comission
Directive 92/69/EEC (which constitutes annex V of council Directive
67/548/EEC). This study complies with the GLP.
Following a preliminary range-finding test fish were exposed, in two
groups of seven, to an aqueaous solution of the test material, at a
single concentration of 100 mg/L for a period of 96 hours at a
temperature of 13.5 to 15.2 °C under semi-static test conditions. The
number of mortalities and any sub-lethal effects of exposure in each
test and control vessel were determined 3 and 6 hours after the start of
exposure and then daily throughout the test until after 96 hours.
The 96 hour LC50 based on nominal test concentration was greater than
100 mg/L and correspondingly the No Observed Effect Concentration was
Analysis of the test preparations at 0, 24 and 96 hours showed measured
test concentrations to range from 91% to 112% of nominal and so the
results are based on nominal test concentrations only.
It was considered unnecessary and unrealistic to test at concentration
in excess of 100 mg/L.
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