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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP STUDY, OECD guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report Date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
directive CE - méthode C1
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
2003-02-13

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: compact
Details on test material:
description: yellow solid block
batch number: 173 jg1
received: 2005-04-29
storage conditions: room temperature in the dark

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
concentration: 100 mg/L, in duplicate

Test solutions

Vehicle:
no
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: none

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
supplier: Brow well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK
acclimatised from 2005-06-01 to 2005-06-13
light cycle: 16 hour light and 8 hours darkness

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
CA 100 mg CaCO3/L
Test temperature:
13.5-15.2 °C
pH:
7.6 to 8.2
Dissolved oxygen:
from 9.4 to 10.2 mg/L
Nominal and measured concentrations:
nominal 100 mg/L
measured: from 90.7 to 110 mg/L
Details on test conditions:
20-litre glass exposure vessels were used for each test concentration.
At the start of the test seven fish were placed in each test vessel at random, in the test preparations. The test vessels were then covered to reduce evaporation and maintained at 13.5 to 15.2°C in a temperature controlled room.
the fish were not individually identified and received no food during exposure.
Any mortalities and sub-lethal effects of exposure were recorded at 3, 6, 24, 48, 72, and 96hours after the start of exposure.
The criteria of death were taken to be the absence of both respiratory movement and response to physical stimulation.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
ca. 100 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
%Concentration loss over test: 9 ... 12

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
the acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-hour LC 50 of greater than 100 mg/L. Correspondingly the No Observed Effect Concentration was 100 mg/L.
Executive summary:

A study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD guidelines for testing of chemicals (1992) n° 203, "fish acute toxicity test" referenced as method C.1 of comission Directive 92/69/EEC (which constitutes annex V of council Directive 67/548/EEC). This study complies with the GLP.

Following a preliminary range-finding test fish were exposed, in two groups of seven, to an aqueaous solution of the test material, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of 13.5 to 15.2 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until after 96 hours.

The 96 hour LC50 based on nominal test concentration was greater than 100 mg/L and correspondingly the No Observed Effect Concentration was 100 mg/L.

Analysis of the test preparations at 0, 24 and 96 hours showed measured test concentrations to range from 91% to 112% of nominal and so the results are based on nominal test concentrations only.

It was considered unnecessary and unrealistic to test at concentration in excess of 100 mg/L.