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EC number: 203-254-2 | CAS number: 104-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Structure-activity relationships of several anisidine and dibenzanthracene isomers in the w/w+ somatic assay of Drosophila melanogaster.
- Author:
- Rosario Rodriguez-Arnaiz, Guadalupe Ordaz Téllez
- Year:
- 2 002
- Bibliographic source:
- Mutation Research 514 (2002) 193–200
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as per given below
- Principles of method if other than guideline:
- Genetic toxicity in vivo test was performed in the somatic w/w+ assay of Drosophila melanogaster employing insecticide-susceptible (IS) and insecticide-resistant (IR) tester strains
- GLP compliance:
- no
- Type of assay:
- somatic mutation and recombination test in Drosophila
Test material
- Reference substance name:
- p-anisidine
- EC Number:
- 203-254-2
- EC Name:
- p-anisidine
- Cas Number:
- 104-94-9
- Molecular formula:
- C7H9NO
- IUPAC Name:
- 4-methoxyaniline
- Details on test material:
- Name of test material (as cited in study report): p- anisidine
- Molecular formula (if other than submission substance): C7-H9-N-O
- Molecular weight (if other than submission substance): 123.1541 g/mol
- Substance type: Organic
- Physical state: Solid
- Purity: No data available
- Impurities (identity and concentrations): No data available
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: One insecticide-susceptible (IS) Leiden Standard (ST) and one insecticide-resistant (IR) Haag 79-R (HG) strain.
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Details on test animals and env conditions
TEST ANIMALS
- Source: No data available
- Age at study initiation: larval stage
- Weight at study initiation: No data available
- Assigned to test groups randomly: [no/yes, under following basis: ] No data available
- Fasting period before study: No data available
- Housing: No data available
- Diet (e.g. ad libitum): No data available
- Water (e.g. ad libitum): No data available
- Acclimation period: No data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25°C
- Humidity (%):60%
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To: No data available
Administration / exposure
- Route of administration:
- other: On Growing cultures of drosophila
- Vehicle:
- No data available
- Details on exposure:
- No data available
- Duration of treatment / exposure:
- During all three instar stages of larval development.
- Frequency of treatment:
- No data available
- Post exposure period:
- No data available
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1,2 and 4 mM
Basis:
no data
- No. of animals per sex per dose:
- No data available
- Control animals:
- yes
- Positive control(s):
- N-nitrosodimethylamine (DMN)
Examinations
- Tissues and cell types examined:
- Compounded eyes of drosophila melangester
- Evaluation criteria:
- Inspected for the occurrence of white in their compound eyes.
- Statistics:
- Chi square for proportions was used for statistical analysis of the data collected
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No data available
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
The end point for the genetic toxicity in vivo for p-anisidine (104-94-9) was found to be negative in Drosophila melanogaster. - Executive summary:
Genetic toxicity in vivo test was performed in the somatic w/w+ assay of Drosophila melanogaster employing insecticide-susceptible (IS) and insecticide-resistant (IR) tester strains.Growing cultures were exposed to compound during all three instar stages of larval development. Two or three separate experiments were conducted with each single chemical at the same exposure dose.The scoring of etherized flies was carried out in a liquid containing 90 parts ethanol, one part Tween-80 and nine parts water. The eyes of adult females were inspected for mosaic light spots under a dissecting microscope at a magnification of 120×, with optical fiber illumination.For an indirect estimation of the genotoxic effectiveness of the chemicals, the frequency of clones per 104cells was calculated .The chi square for a proportion was used for statistical analysis.
From the above data analysis it was found thatthe end point for the genetic toxicity in vivo for p-anisidine (104-94-9) was found to be negative in Drosophila melanogaster.
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