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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: gene mutation
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Structure-activity relationships of several anisidine and dibenzanthracene isomers in the w/w+ somatic assay of Drosophila melanogaster.
Author:
Rosario Rodriguez-Arnaiz, Guadalupe Ordaz Téllez
Year:
2002
Bibliographic source:
Mutation Research 514 (2002) 193–200

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as per given below
Principles of method if other than guideline:
Genetic toxicity in vivo test was performed in the somatic w/w+ assay of Drosophila melanogaster employing insecticide-susceptible (IS) and insecticide-resistant (IR) tester strains
GLP compliance:
no
Type of assay:
somatic mutation and recombination test in Drosophila

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of test material (as cited in study report): p- anisidine
- Molecular formula (if other than submission substance): C7-H9-N-O
- Molecular weight (if other than submission substance): 123.1541 g/mol
- Substance type: Organic
- Physical state: Solid
- Purity: No data available
- Impurities (identity and concentrations): No data available

Test animals

Species:
Drosophila melanogaster
Strain:
other: One insecticide-susceptible (IS) Leiden Standard (ST) and one insecticide-resistant (IR) Haag 79-R (HG) strain.
Sex:
male/female
Details on test animals and environmental conditions:
Details on test animals and env conditions
TEST ANIMALS
- Source: No data available
- Age at study initiation: larval stage
- Weight at study initiation: No data available
- Assigned to test groups randomly: [no/yes, under following basis: ] No data available
- Fasting period before study: No data available
- Housing: No data available
- Diet (e.g. ad libitum): No data available
- Water (e.g. ad libitum): No data available
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25°C
- Humidity (%):60%
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available

IN-LIFE DATES: From: To: No data available

Administration / exposure

Route of administration:
other: On Growing cultures of drosophila
Vehicle:
No data available
Details on exposure:
No data available
Duration of treatment / exposure:
During all three instar stages of larval development.
Frequency of treatment:
No data available
Post exposure period:
No data available
Doses / concentrations
Remarks:
Doses / Concentrations:
1,2 and 4 mM
Basis:
no data
No. of animals per sex per dose:
No data available
Control animals:
yes
Positive control(s):
N-nitrosodimethylamine (DMN)

Examinations

Tissues and cell types examined:
Compounded eyes of drosophila melangester
Evaluation criteria:
Inspected for the occurrence of white in their compound eyes.
Statistics:
Chi square for proportions was used for statistical analysis of the data collected

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
not specified
Vehicle controls validity:
not specified
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data available

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
The end point for the genetic toxicity in vivo for p-anisidine (104-94-9) was found to be negative in Drosophila melanogaster.
Executive summary:

Genetic toxicity in vivo test was performed in the somatic w/w+ assay of Drosophila melanogaster employing insecticide-susceptible (IS) and insecticide-resistant (IR) tester strains.Growing cultures were exposed to compound during all three instar stages of larval development. Two or three separate experiments were conducted with each single chemical at the same exposure dose.The scoring of etherized flies was carried out in a liquid containing 90 parts ethanol, one part Tween-80 and nine parts water. The eyes of adult females were inspected for mosaic light spots under a dissecting microscope at a magnification of 120×, with optical fiber illumination.For an indirect estimation of the genotoxic effectiveness of the chemicals, the frequency of clones per 104cells was calculated .The chi square for a proportion was used for statistical analysis.

From the above data analysis it was found thatthe end point for the genetic toxicity in vivo for p-anisidine (104-94-9) was found to be negative in Drosophila melanogaster.