Registration Dossier

Ecotoxicological information

Long-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p-Anisidine
- Molecular formula: C7-H9-N-O
- Molecular weight: 123.1541g/mol
- Smiles notation: c1(ccc(cc1)N)OC
- InChl: 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
- Common name: Japanese killifish

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Test temperature:
24±1 degreeC
Nominal and measured concentrations:
100, 50, 25.0, 12.5 mg/L (geometric ratio 2.0), control
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: test liquid volume 3.6 L per concentration (1.8 L per vessel)
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): continuous supply system of test solution
- No. of organisms per vessel: 20 per concentration (10 per vessel)
- No. of vessels per concentration (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
70.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95 % CL 50 - 100 mg/L

Applicant's summary and conclusion

Conclusions:
LC50 values of p-Anisidine in Oryzias latipes were found to be >100mg/l for 14 days and 70.7mg/l for 21 days based on effect on mortality while
NOEC was found to be 12.5mg/l for 21 days exposure.
Executive summary:

Toxicity study of p-Anisidine in Oryzias latipes was conducted under flow –through condition for an exposure period of 14 and 21 days at dose concentrations of 0, 12.5, 25, 50 and 100 mg/L.

LC50 values of p-Anisidine in Oryzias latipes were found to be >100mg/l for 14 days and 70.7mg/l for 21 days based on effect on mortality while NOEC was found to be 12.5mg/l for 21 days exposure.