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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p-Anisidine
- Molecular formula (if other than submission substance): C7-H9-N-O
- Molecular weight (if other than submission substance): 123.1541g/mol
- Smiles notation (if other than submission substance): c1(ccc(cc1)N)OC
- InChl (if other than submission substance): 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Separation method (e.g. HPLC, GC): HPLC
- other: measurement frequency: start of exposure, before renewal of test solution

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
20±1 degreeC
Nominal and measured concentrations:
450, 100, 22.2, 4.94, 1.10, 0.244 mg/L (geometric ratio 4.5), control
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume:test liquid volume 100 mL per vessel
- Renewal rate of test solution (frequency/flow rate): replace the total amount of test solution after 24 hours
- No. of organisms per vessel: 20 per concentration (5 per vessel)
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks:
Immobilization
Remarks on result:
other: 95 % CL 2.76 - 6.13 mg/L

Applicant's summary and conclusion

Conclusions:
EC50 for p-Anisidine in Daphnia magna was found to be 4.12 mg/l, when exposed for 48h under semi-static condition.
Executive summary:

In the study Short-term toxicity to aquatic invertebrates for p-Anisidine, Daphnia magna was exposed at nominal and measured concentrations of 450, 100, 22.2, 4.94, 1.10, 0.244 mg/L for duration of 48 hr. Immobilization was observed at concentration of 4.12 mg/l. Therefore EC50 for p-Anisidine

was considered to be 4.12 mg/l in Daphnia magna when exposed for 48h under semi-static condition.