p-anisidine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

p-anisidine

EC Number:

203-254-2

EC Name:

p-anisidine

Cas Number:

104-94-9

Molecular formula:

C7H9NO

IUPAC Name:

4-methoxyaniline

Details on test material:

- Name of test material: p-Anisidine
- Molecular formula: C7-H9-N-O
- Molecular weight: 123.1541g/mol
- Smiles notation: c1(ccc(cc1)N)OC
- InChl: 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Common name: Water flea

Study design

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Test temperature:

20±1 degreeC

Nominal and measured concentrations:

2.0, 1.0, 0.50, 0.250, 0.125 mg/L (geometric ratio 2.0), control

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume:test liquid volume ca.1.8 L per vessel
- Renewal rate of test solution (frequency/flow rate): continuous supply system of test solution
- No. of organisms per vessel: 10 per vessel (40 per concentration)
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.298 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

inhibition

Remarks on result:

other: 95 % CL 0.288 - 0.312 mg/L

Applicant's summary and conclusion

Conclusions:

EC50 value for p-Anisidine in Daphnia magna for 21 days under flow –through condition was found to be 0.298 mg/l on basis of inhibition of reproduction whereas the NOEC was found to be 0.125 mg/l.

Executive summary:

In the study Long-term toxicity to aquatic invertebrates for p-Anisidine, Daphnia magna were exposed at nominal and measured concentration: 2.0, 1.0, 0.50, 0.250, 0.125, 0.0 mg/L for 21 days. Inhibition in reproduction was observed in Daphnia magna. Therefore EC50 value for p-Anisidine in Daphnia magna for 21 days under flow –through condition was found to be 0.298 mg/l on basis of inhibition of reproduction whereas the NOEC was found to be 0.125 mg/l.