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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material: p-Anisidine
- Molecular formula: C7-H9-N-O
- Molecular weight: 123.1541g/mol
- Smiles notation: c1(ccc(cc1)N)OC
- InChl: 1S/C7H9NO/c1-9-7-4-2-6(8)3-5-7/h2-5H,8H2,1H3
- Substance type: Organic
- Physical state: Grey to brown crystalline powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Common name: Water flea

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Test temperature:
20±1 degreeC
Nominal and measured concentrations:
2.0, 1.0, 0.50, 0.250, 0.125 mg/L (geometric ratio 2.0), control
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume:test liquid volume ca.1.8 L per vessel
- Renewal rate of test solution (frequency/flow rate): continuous supply system of test solution
- No. of organisms per vessel: 10 per vessel (40 per concentration)
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light - 8 hours dark
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.298 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks:
inhibition
Remarks on result:
other: 95 % CL 0.288 - 0.312 mg/L

Applicant's summary and conclusion

Conclusions:
EC50 value for p-Anisidine in Daphnia magna for 21 days under flow –through condition was found to be 0.298 mg/l on basis of inhibition of reproduction whereas the NOEC was found to be 0.125 mg/l.
Executive summary:

In the study Long-term toxicity to aquatic invertebrates for p-Anisidine, Daphnia magna were exposed at nominal and measured concentration: 2.0, 1.0, 0.50, 0.250, 0.125, 0.0 mg/L for 21 days. Inhibition in reproduction was observed in Daphnia magna. Therefore EC50 value for p-Anisidine in Daphnia magna for 21 days under flow –through condition was found to be 0.298 mg/l on basis of inhibition of reproduction whereas the NOEC was found to be 0.125 mg/l.