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EC number: 222-720-6
CAS number: 3586-55-8
Results of main test:
Cell concentrations (mean values)[104cells/mL]
Percent of control
22.1 – 23.2 °C
reaction products of ethylene glycol with paraformaldehydeto the freshwater
green algae Desmodesmus subspicatus
was studied in a
test according to the OECD Guideline 201.
algae were exposed to 5 concentrations of the test substance, ranging
from 0.625 to 10 mg/L. The initial cell concentration was
8.1 • 104cells/mL
instead of ca. 104 cells/mL recommended by the Guideline.
In the test medium, NaHCO3was
twice as concentrated (100 mg/L) as indicated in the Guideline (50 mg/L).
concentrations were tested using 5 replicates. The test was performed at
22.1 – 23.2 °C in a medium according to the OECD Guideline. The cells
were counted after 0, 24, 48, and 72 hours, the pH value was measured at
the start and end of the test.
validity criteria according the test Guideline are met within the study.
concentration-response relationship can be established from the study
results. Both, the initial cell concentration of the algae and NaHCO3concentration
were above the values recommended by the Guideline. The latter deviation
is justified as the higher concentration of hydrogencarbonate should
stabilize the pH value.
substance EGForm hydrolyses rapidly and completely in the test medium,
forming ethylene glycol and formaldehyde. Formaldehyde is expected to
dominate the toxicity of the EGForm
in aqueous solutions.
Formaldehyde is expected to be not volatile from the test solution, and
no losses due to adsorption are expected.
of formaldehyde was unsuccessful, as it could not be detected neither in
the t0 nor in the t72h samples. This behaviour was
not observed in test solutions of the respective tests with daphnia and
fish. There is no explanation for the negative result. A possible reason
for the disappearance of the test substance may be a reaction with
components of the test solution (possibly ammonium).
in the present test a clear concentration-effect curve could be
obtained, which clearly indicates the presence of the test substance.
This assumption, i.e. presence of the test substance, is supported by
the monitoring data obtained in the respective fish and daphnia tests.
Therefore, the effective concentrations can be considered to be
identical with the nominal concentrations.
toxicity was found with EC50s of 3.48 mg/L and 4.62 mg/L for
biomass production and growth rate, respectively. The NOEC based on the
growth rate was determined to be 1.11 mg/L.
Monitoring in the algae test was
unsuccessful, probably because of reaction of formaldehyde with a
component of the test medium. Because of the clear dose-effect
relationship obtained in the test and the positive monitoring results in
the fish and daphnids test, the nominal concentrations in the algae test
are considered as effective concentrations.
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