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EC number: 222-720-6
CAS number: 3586-55-8
of determination ofthe
test substance in the test medium:
Sampling time [h]
Nominal Conc. [mg/L]
Measured Conc. [mg/L]
results in the table were not corrected for the recoveries obtained by
the analytical methodhe mean recovery rate was 100%.
stated that the test solutions showed no significant loss of the test
substance. Therefore, nominal concentrations can be considered as the
note: Some individually-measured concentrations deviate by more than 20%
from the nominal. However, the mean values were within 20% of the
nominal concentrations. A
general trend towards loss
of formaldehyde in 24 h old solutions, compared to freshly prepared
media, cannot be observed. Therefore, the demands of the Guideline
(stability ≥ 80% of nominal) can be regarded as fulfilled.
of main test:
pH freshly prepared
pH after 24 h
24 h test temperature was lower than the range proposed by the Guideline
(21-25°C). This deviation is not considered by the authors to have an
influence on the integrity of the study.
The reaction products of ethylene
glycol with paraformaldehyde were tested for acute toxicity towards
Danio rerio according to OECD-Test Guideline 203. 7 fish were exposed in
a semistatic system to 5 concentrations of the test substance between
6.25 and 100 mg/L and a control group. The test solutions were prepared
in drinking water and renewed daily. Test duration was 96 hours and the
test solutions were examined daily for dead fish. Oxygen concentrations,
temperature and pH of the medium were measured in the same intervals.
Analysis of the formaldehyde concentrations was performed daily by HPLC.
The test substance was found to be
acutely harmful towards Danio rerio. All results refer to the nominal
concentrations due to formaldehyde concentration being shown stable
within the incubation period.
A clear concentration-response
relationship can be established from the study results. Lethal effects
were observed only at a concentration of 100 mg/L. The next
concentration of the geometric series exhibited no toxicity (manifest as
mortality). Therefore, the LC50 was calculated as the geometric mean
between LC0 and LC100 representing 71 mg/L of the test substance.
The test substance EGForm hydrolyses
rapidly and completely in the test medium, forming ethylene glycol and
formaldehyde. Formaldehyde is expected to dominate the toxicity of
EGForm in aqueous solutions. Dissolved formaldehyde is not expected to
be volatile, and no losses due to adsorption are to be expected.
Analysis of the formaldehyde
concentrations during the test proved that no significant losses
occurred with respect to the mean measured values. Individual results,
however, are deviating by more than 20% of the nominal concentration. A
general trend towards loss of formaldehyde in 24 h old solutions,
compared to freshly prepared media, cannot be observed. Therefore, the
effective concentrations can be considered to be equal to the nominal
a study on the acute toxicity of the test substance towards fish the LC50
was determined to be 71 mg/L employing the zebrafish (Danio
rerio) as the test organism.
The acute 96 h LC50 value of ethylene glycol
to P. promelas was determined to 72860 mg/L.
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