Cycloocta-1,5-diene

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity

Author:

Sparnins VL, Lam LKT and Wattenberg LW

Year:

1984

Bibliographic source:

Biochem. Biophys. Res. Commun. 120, 2, 637-640

Materials and methods

Principles of method if other than guideline:

The effects of cyclic 12-, 8- and 6-carbon  compounds  (incl. 1,5-cyclooctadiene) on the glutathione S-transferase  (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the  forestomach of female ICR/Ha mice were investigated.

GLP compliance:

no

Type of method:

in vivo

Endpoint addressed:

basic toxicokinetics

Test material

Test material information

Constituent 1

Reference substance name:

Cycloocta-1,5-diene

EC Number:

203-907-1

EC Name:

Cycloocta-1,5-diene

Cas Number:

111-78-4

Molecular formula:

C8H12

IUPAC Name:

(1Z,5Z)-cycloocta-1,5-diene

Details on test material:

1,5-Cyclooctadiene from Aldrich Chemical Company, purity not reported

Test animals

Species:

mouse

Strain:

ICR

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ORGANISM
- Female ICR/Ha mice
- Age: 7 weeks
- Number: 5-10 animals per group
- Control: diet

Administration / exposure

Route of administration:

oral: feed

Vehicle:

unchanged (no vehicle)

Details on exposure:

TREATMENT - 30 or 50 µmol/g in diet for 2 weeks

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

2 weeks

Frequency of treatment:

every time

Post exposure period:

no

Doses / concentrations

Remarks:

Doses / Concentrations:
30 or 50 µmol/g diet
Basis:
nominal in diet

No. of animals per sex per dose:

5 - 10

Control animals:

yes, plain diet

Details on study design:

no further details

Examinations

Examinations:

EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate

Positive control:

no

Results and discussion

Details on results:

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds 
were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.

Any other information on results incl. tables

None of the compounds elicited increased GST activity in the forestomach.  

C6 ring compounds showed no significant effect. C12 ring compounds were  more 

effective than C8 ring compounds with a decrease in activity in the  order:
unsaturated > epoxide > alcohol > saturated.
-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                1.96 +- 0.16           0.61 +- 0.04
1,5-Cyclooctadiene  2.75 +- 0.26*          0.76 +- 0.03*    
                                    * = p <0.005

Applicant's summary and conclusion

Conclusions:

1,5-cyclooctadiene was few effective in producing increased GST activity in the liver.

Executive summary:

Glutathione S-transferase is considered as a major detoxification system  which catalyzes conjugation of electrophilic compounds, e.g. chemical  carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon  compounds  (incl. 1,5-cyclooctadiene) on the

glutathione S-transferase  (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the  forestomach of female ICR/Ha mice were investigated.

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed 

no significant effect. C12 ring compounds were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.