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Registration Dossier
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EC number: 203-907-1 | CAS number: 111-78-4
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Reference Type:
- publication
- Title:
- Robust summary and test plan for 1,5-cyclooctadiene with cover letter dated 11 Dec 2002
- Author:
- DuPont Safety, Health & Environmental Excellence Center, Wilmington (Del., USA)
- Year:
- 2 002
- Bibliographic source:
- Quelle U.S. EPA 37 pp
Materials and methods
- Principles of method if other than guideline:
- Method: other: See Test Conditions: standard acute inhalation toxicity study; nose-only
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Cycloocta-1,5-diene
- EC Number:
- 203-907-1
- EC Name:
- Cycloocta-1,5-diene
- Cas Number:
- 111-78-4
- Molecular formula:
- C8H12
- IUPAC Name:
- (1Z,5Z)-cycloocta-1,5-diene
- Details on test material:
- Test substance: other TS: 1,5-cyclooctadiene, purity > 99%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD(R) BR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ORGANISMS:
- Source:
- Age: approximately 8 weeks
- Weight at study initiation: 268-292 g
- Number of animals: 6
- Controls: no
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: houseline air
- Details on inhalation exposure:
- ADMINISTRATION:
- Type of exposure: single nose-only exposure
- Concentrations: measured at approximately 15-minute intervals by GC
- Type or preparation of particles: No particles. Chamber atmospheres were generated by vaporizing the test substance in a heated nitrogen stream and dilution with houseline air.
- Temperature, humidity in chamber: 23 +/- 2 °C, 50 +/- 10 % - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Concentrations was measured at approximately 15-minute intervals by GC
- Duration of exposure:
- 4 h
- Concentrations:
- 1400, 2700, or 4300 ppm = 6.30, 12.1, or 19.3 mg/l
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- EXAMINATIONS:
mortality and response to stimuli during exposure; clinical signs and mortality immediately following exposure; body weight and clinical signs daily
thereafter for 14 days (during weekends only when warranted by the health status of the rats). - Statistics:
- Evaluation with simple statistics at p <= 0.05.
Results and discussion
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- other: ALC (approximate lethal concentration)
- Effect level:
- ca. 12 - ca. 19 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- - 1400 ppm: 0/6
- 2700 ppm: 1/6 night after exposure
- 4300 ppm: 4/6 during exposure - Clinical signs:
- other: - Response to external sound stimuli: Absent for all animals after 3 hours exposure - Immobility: 2 surviving rats at 4300 ppm and 4 rats at 2700 ppm - Ataxia: 3 rats at 1400 ppm - Ocular discharge, ruffled fur, or stained perineum: Up to 4 days follow
- Body weight:
- Body weights: Losses of 4-14% were recorded the day after exposure, followed by body weight gains in all animals.
- Gross pathology:
- no data
- Other findings:
- no other findings
Any other information on results incl. tables
no further results
Applicant's summary and conclusion
- Conclusions:
- Under the present test conditions, test item is considered slightly toxic in an acute inhalation toxicity study.
- Executive summary:
Three groups of 6 male rats each were exposed nose-only for a single, 4 hour period to vapors of the test item in air at chamber vapor concentrations of 1400, 2700 or 4300 ppm. Mortality was 0/6, 1/6 and 4/6 at 1400, 2700 and 4300 ppm respectively. By 3 hours into the exposure all rats failed to respond to external sound stimuli. At the end of exposure, the 2 rats at 4300 ppm and 4 rats at 2700 ppm were immobile. Threee rats at 1400 ppm exhibited ataxia. Other clinical signs of toxicity observed after exposure: ocular discharge, lethargy, irregular respiration. Ocular discharge, ruffled fur or stained perineum were observed up
to 4 days following exposure.
On an acute inhaltion basis, test item is considered slightly toxic.
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