Cycloocta-1,5-diene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985-10-01 to 1985-10-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods with acceptable restrictions (no solvent control, no analytical monitoring)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 15

Principles of method if other than guideline:

Method: DIN 38412 part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cycloocta-1,5-diene

EC Number:

203-907-1

EC Name:

Cycloocta-1,5-diene

Cas Number:

111-78-4

Molecular formula:

C8H12

IUPAC Name:

(1Z,5Z)-cycloocta-1,5-diene

Details on test material:

- cis,cis-1,5-cyclooctadiene of Hüls AG
- purity not reported

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dispersion: MARLOWET EF (castor oil, ethoxylated, 40 EO) was added as solubilizer
- Concentration of vehicle/ solvent: 10 g test substance / l,  concentration of solubilizer not reported

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISMS
- Strain: Leuciscus idus melanotus HECKEL
- Supplier: Fischzucht Eggers, Hohenwestedt (Germany)
- Wild caught: no
- Age/size/weight/loading: length 6 cm +/- 2 cm
- Feeding: daily 3 % of body weight TetraMin
- Pretreatment: single treatment with Zephirol 1:50,000 for 1 hour  followed by 14 days under quarantine
- Feeding during test: no

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

- ca. 15 degree dH

Test temperature:

- Test temperature: 20 +/- 1 degree C

pH:

- pH: 7.3-7.7

Dissolved oxygen:

- Dissolved oxygen: 8.2-8.7 mg/l

Nominal and measured concentrations:

- Concentrations: 6, 8, 10, 13, 16 mg/l (nominal)

Details on test conditions:

DILUTION WATER
- Source: dechlorinated drinking water
- Aeration: continuous

TEST SYSTEM
- Renewal of test solution: daily
- Exposure vessel type: 10 l solution in 18 l aquarium
- Number of replicates, fish per replicate: 1, 10
- Photoperiod: 8 hours light / 16 hours dark

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

6 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

9.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 6.4-13.4 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

16 mg/L

Details on results:

LC0 (48 h): 6 mg/L (highest concentration at which mortality was <10%)
LC100 (48 h): 16 mg/L (lowest concentration at which mortality was 100%)

Any other information on results incl. tables

Sublethal observations / clinical signs:

- Effect data (Mortality): after 48 hours
   6 mg/l:   0 % dead
   8 mg/l:  50 % dead
  10 mg/l:  60 % dead
  13 mg/l:  80 % dead
  16 mg/l: 100 % dead
  95 % confidence interval of LC50: 6.4 - 13.8 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (48 hours) was determined as 9.4 mg/L indicating that the test substance may be toxic to fish.

Executive summary:

The acute toxicity of 1,5 -cyclooctadiene on Leuciscus idus was tested in a semi-static 48h-test by Hüls AG (1995) according to DIN 38412, part 15. Five concentrations ranging from 6.0 to 16.0 mg/L were tested. The following endpoint was derived:

LC50 (48 hours):       9.4 mg/L (CL: 6.4 -13.4 mg/L)

The study was assessed as "reliable with restriction".