Cycloocta-1,5-diene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997-12-17 to 1997-12-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cycloocta-1,5-diene

EC Number:

203-907-1

EC Name:

Cycloocta-1,5-diene

Cas Number:

111-78-4

Molecular formula:

C8H12

IUPAC Name:

(1Z,5Z)-cycloocta-1,5-diene

Details on test material:

Test substance: cis,cis-1,5-cyclooctadiene (Hüls AG)
Batch B803, produced 10 October 1997
Purity 99.6 % (GC-FID area)
Sample No. 1672/971103
Sample ID 0637/81865

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples for test substance analysis were taken at the start (0 hours) and at the end of the test (48 hours)

Test solutions

Vehicle:

no

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 1 g/l stirred in deionized water for 18 hours and  filtered, no vehicle or solvent; analytical concentration 87.3 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISMS
- Strain: Daphnia magna Straus, clone 5
- Source/supplier: Hüls AG (inhouse)
- Breeding method: in 1 l beakers with M4 medium (Elendt, 1990), water renewal each 2-3 days, isolation of offspring for further breeding each ca. 
4 weeks
- Age: < 24 hours
- Feeding: Scenedesmus subspicatus, daily as much as consumed
- Pretreatment: Filtration of adults 24 h prior to testing - Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 1 degree C

pH:

7.7 - 8.1

Dissolved oxygen:

7.5 - 8.4 mg/l

Nominal and measured concentrations:

0.79, 1.1,  1.7,  2.6,  3.9, 6.1, 8.7 mg/l (nominal)   
0.43, 0.72, 1.1,  1.8,  2.8, 4.8, 7.7 mg/l (analysis, 0 h)   
0.37, 0.51, 0.63, 0.95, 1.5, 2.6, 3.1 mg/l (analysis, 48 h)

Details on test conditions:

TEST SYSTEM
- Renewal of test solution: no
- Exposure vessel type: round-bottom test tubes with 10 ml test solution  and rubber plug to avoid volatilization
- Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
- Control group: 2 reference substance controls (1.0 and 2.0 mg/l), one  blank
- Adjustment of pH: no
- Photoperiod: dark

DILUTION WATER
- Source: Synthetic: CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 6 H2O: 114 mg/l; NaHCO3: 65 mg/l; KCl: 6 mg/l
- Ca/Mg ratio: 4:1 - Na/K ratio: 10:1
- Aeration: no

TEST PARAMETER: immobilisation
- the mean of the analytical values at 0 and 48 hours was used for the evaluation.

Reference substance (positive control):

yes

Remarks:

potassium dichromate, CAS RN 7778-50-9

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.83 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Due to the steep dose response response curve the EC50-value was determined graphically.
EC0: highest concentration tested resulting in <10% effects
EC100: lowest concentration tested resulting in 100% effects

Results with reference substance (positive control):

- Concentrations: 1.0 mg/l / 2.0 mg/l
- Results: 35 % immobile / 100 % immobile (24 hours)

Any other information on results incl. tables

RESULTS: EXPOSED, CONTROL
- Effect data (Immobilisation): No. of immobile daphnia
  Control:    0 (24 h) /   0 (48 h)
  0.40 mg/l:  0 (24 h) /   0 (48 h) 
  0.61 mg/l:  0 (24 h) /   0 (48 h)
  0.83 mg/l:  0 (24 h) /   0 (48 h) 
  1.3  mg/l: 14 (24 h) /  15 (48 h) 
  2.0  mg/l: 20 (24 h) /  20 (48 h) 
  3.5  mg/l: 20 (24 h) /  20 (48 h) 
  4.9  mg/l: 20 (24 h) /  20 (48 h) 
  EC50 (24 hours) = EC50 (48 hours) = 1.1 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 (48 hours) was determined as 1.1 mg/L indicating that the test substance may be toxic to aquatic invertebrates.

Executive summary:

The acute toxicity of 1,5 -cyclooctadiene on Daphnia magna was investigated in a 48 -h test by Hüls AG (1998) according to EU method C.2. Seven concentrations ranging from 0.4 mg/L to 4.9 mg/L (mean values of measured concentrations) plus controls were tested. The following endpoint was derived:

EC50 (48 hours): 1.1 mg/L

The study was assessed as "reliable without restrictions".