Cycloocta-1,5-diene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-04-19 to 1983-05-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cycloocta-1,5-diene

EC Number:

203-907-1

EC Name:

Cycloocta-1,5-diene

Cas Number:

111-78-4

Molecular formula:

C8H12

IUPAC Name:

(1Z,5Z)-cycloocta-1,5-diene

Details on test material:

1,5-Cyclooctadiene of Hüls AG; Purity 99.5 %

Test animals

Species:

rabbit

Strain:

other: Small white Russian, Chbb-SPF

Details on test animals or test system and environmental conditions:

TEST ANIMALS: 
- Strain: Small white Russian, Chbb-SPF
- Sex: male and female
- Source: F. Winkelmann, Borchen (Germany)
- Weight at study initiation: 2.3 - 2.7 kg
- Number of animals: 3 males, 3 females
- Feed: K4 complete feed for rabbits (Ssniff, Soest, Germany);
- Water: tap water ad libitum
Environmental conditions:
- Room temperature: 20°C (+/- 1°C);
- Humidity: 60% (+/- 5%);
- Air change: 15 times/hour;
- Light: 12 hour light/dark rhythm;

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

other: no vehicle

Controls:

not required

Amount / concentration applied:

undiluted

Duration of treatment / exposure:

4 hour(s)

Observation period:

20 days

Number of animals:

6

Details on study design:

ADMINISTRATION/EXPOSURE 
- Area of exposure: 6 cm2
- Total volume applied: 0.5 ml
- Occlusion: mull patch, polyethylene film, elastic dressing
- Removal of test substance: careful washing with deionized water
- Post exposure period: 20 days
EXAMINATIONS
- Scoring system: OECD; evaluation: VCI notes on safety data sheet
- Examination time points: 1, 24, 48, 72 hours, 6, 8, 10 and 20 days  
after patch removal; evaluation based on readings 1-4

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 1, 24, 48, 72 h

Score:

1

Max. score:

8

Reversibility:

fully reversible within: 20 days

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24, 48, 72 h

Score:

1.06

Max. score:

4

Reversibility:

fully reversible within: 20 days

Results 3

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24, 48, 72 h

Score:

0

Max. score:

4

Irritant / corrosive response data:

AVERAGE SCORE
- Overall irritation index: 1.0
- Erythema: 1.06
- Edema: 0
REVERSIBILITY: complete within 20 days.

Other effects:

OTHER EFFECTS: eschar formation (days 6-10)

Any other information on results incl. tables

no further remarks

Applicant's summary and conclusion

Conclusions:

According to this study, the test item showed slightly irritant effect on the skin of male and female rabbits.

Executive summary:

To test the acute skin irritant effect the test item was applied to the shaven dorsal skin of 3 female and 3 male rabbits. The product was administered undiluted and the time of exposure in the patch test was 4 hours. The dermal reactions were fully reversible within 20 days. According to the study, the test item 1,5-Cyclooctadiene showed slightly irritant effect on the skin of male and female rabbits.