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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The paper was published in Russian but an English translation was presented by RIFM. The purity of the test substance is unknown, the system of vapour generation was not described in details and control animals were not mentioned. Furthermore, this study is performed on mice and only after 2 hours of exposure. For these reasons, a reliability 4 was assigned. The GLP were not mentioned but the study was performed before the GLP standard was established.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Monographs on fragrance raw materials
Author:
Opdyke DL, Letizia C.
Year:
1982
Bibliographic source:
Food Chem Toxicol, 20, 697-701.
Reference Type:
publication
Title:
Toxicology of guaiacol vapors and of its resin
Author:
Ostrovsky M.M.
Year:
1964
Bibliographic source:
Journal Gigiena i Sanitariya, 29, 85-88. (Russian)
Reference Type:
review article or handbook
Title:
Gaiacol
Author:
RIFM ([url]: http://rifm.org/nd/mono.cfm?CASNumber=90-05-1)
Year:
1982
Bibliographic source:
Monograph: 9 (Published 1982: FCT,v20,p697, Special Issue VI)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acute toxicity inhalation
GLP compliance:
not specified
Test type:
other: acute toxicity inhalation
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Guaiacol
EC Number:
201-964-7
EC Name:
Guaiacol
Cas Number:
90-05-1
Molecular formula:
C7H8O2
IUPAC Name:
2-methoxyphenol
Details on test material:
The origin of guaiacol was not reported and its purity was unknown.

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
other:
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
2 h
Concentrations:
2.03, 3.6, 4, 6.13, 6.42, 7.57, 7.74, 7.76, 9.53, 12.64, 17.19 mg/L
No. of animals per sex per dose:
70 mice in the experiment
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LC50
Effect level:
7.57 mg/L air
Exp. duration:
2 h
Remarks on result:
other: The survival rate of the animals was the criterion for evaluating toxicity.
Sex:
not specified
Dose descriptor:
LC50
Effect level:
3.78 mg/L air
Exp. duration:
4 h
Remarks on result:
other: Calculated
Mortality:
From 7.76 mg/L guaiacol vapours to 12.64 mg/L, the majority of animals died. At 17.19 mg/L, all the animals died and it was considered as LC100.
Clinical signs:
other: In the initial period of the substance effects, all the mice showed slight inflammation of respiratory tract and eye mucous membranes. These symptoms were accompanied by short-term nervousness, followed by little activity. These clinical signs were rever
Gross pathology:
Guaiacol vapors showed also damage to the vascularization of the internal organs and bleeding into the membrane and brain matter. Dystrophic
changes were rarer: protein dystrophy of the heart muscle and focal fat of the parenchyma of the liver.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Executive summary:

In an acute inhalation toxicity study (Ostrovsky, 1964), groups of mice were exposed by inhalation route to guaiacol for 2 hours at concentrations of 2.03, 3.6, 4, 6.13, 6.42, 7.57, 7.74, 7.76, 9.53, 12.64, 17.19 mg/L.

The LC50 = 7.57 mg/L for 2 hours (calculated as 3.78 mg/L for 4 hours) .

Based on this data, no conclusion on guaiacol classification can be drawn for this endpoint, since this study was performed in mice instead of rats and during only 2 hours instead of 4 as required in the OECD guideline.

Guaiacol was officially classified in the Regulation (EC) n°1272/2008 Annex VI table 3 and was not classified for acute toxicity by inhalation.