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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No information was available about the test substance. No information was available about the control group and the test concentrations.
Qualifier:
equivalent or similar to
Guideline:
other: screening test
Deviations:
not specified
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: no data
- Age at study initiation: 12 +/- 12 hours old.
- Method of breeding: no data
- Feeding during test:
- Food type: the food suspension was prepared by weighing 0.25 g of powdered grass and 5 g of enriched trout fry granules. This mixture was added to 125 ml of Lake Superior water and mixed vigorously in a blender for 5 min. An additional 25 ml of lake water was used to rinse out the blender. The suspension was then filtered through N° 20 bolting cloth.
- Amount: 0.2 ml/ 200 ml of suspension
- Frequency: at the beginning of each test

ACCLIMATION: no data
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
4 d
Test temperature:
18 +/- 1°C
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: 4 beakers filled with 200 ml of solution each
- Aeration: no data
- No. of organisms per vessel: 5
- No. of vessels per concentration: 3 or more
- No. of vessels per control: no data
- No. of vessels per vehicle control: no data

WATER PARAMETERS
- Source/preparation of dilution water: Non-filtered and non-chlorinated Lake Superior water from the Duluth National Water Quality Laboratory (NWQL).

TEST MEDIUM PARAMETERS: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod
- Light intensity: fluorescent bulbs, a combination of Durotest (Optima FS) and wide spectrum Grow-lux bulbs, provided an intensity of 475 foot-candles at the air-water interface.
- Test temperature: 18 +/- 1°C
- pH: no observed pH change
- dissolved oxygen: no data

EFFECT PARAMETERS MEASURED: mortality; the daphnia were counted each day for 4 days and the number of survivors recorded.
TEST CONCENTRATIONS: four dose levels, without any details
Reference substance (Positive control): no data
Duration:
4 d
Dose descriptor:
EC50
Effect conc.:
25.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
LC50 were calculated on 48 hours but exposure/ observation period was continued on 4 days.
Reported statistics and error estimates:
For each compound a graph was prepared plotting log percent survivors vs. time in hours. From this plot it was possible to determined the percent survivors, for each dose level at 48 h. The 48-h percent survival figures were converted to probit transformation tables. These values were plotted vs. log molar concentration. The log LC50 molar concentration can then be found by observing the log molar concentration value which corresponds to a probit value of 5. The probit values and log molar concentration values were introduced into a least squares computer program which calculated the LC50 and also gave the correlation of the line to the data points.
This method for determining the 48-h LC50 value, has the advantage of a 4-day observation period. The inconsistencies which arise when animals are counted only once (at 48h) are thereforeaveraged out, resulting in greater reproductibility.
Validity criteria fulfilled:
not specified
Conclusions:
Harmful for aquatic organisms.
Executive summary:

The 48-hr acute toxicity of guaiacol to Daphnia magna was studied under static conditions. Daphnids were exposed to test chemical (concentrations not specified) for 96 hr. Mortality was observed daily. The 48-hour LC50 was determined graphically by the method of log probit and was found to be 25.9 mg/L.

Based on the results of this study, guaiacol would be classified as harmful to Daphnia magna in accordance with the classification system of the EU.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study was conducted following a standardised method.
Qualifier:
according to
Guideline:
other: AFNOR T90301 of april 1974, modified according to AFNOR T95B DOC 19
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
- Method: The test substance was diluted in bidistilled water at the concentration slightly higher than the expected toxicity threshold.
- Controls: no data
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Details on test conditions:
TEST SYSTEM: no data
WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted River water
TEST MEDIUM PARAMETERS: no data
OTHER TEST CONDITIONS: no data
EFFECT PARAMETERS MEASURED: immobility
TEST CONCENTRATIONS: no data
Duration:
24 h
Dose descriptor:
IC50
Effect conc.:
63 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 45-87
Reported statistics and error estimates:
no
Validity criteria fulfilled:
not specified
Executive summary:

The 24-hr-acute toxicity of guaiacol to Daphnia magna was studied under static conditions following the AFNOR T90301 of april 1974, modified according to AFNOR T95B DOC 19 . Daphnids were exposed to test chemical (concentrations not detailed), and Mortality/immobilization were observed.  The 24- hour IC50 was 63 mg/L. 
 
Based on the results of this study, gaiacol would
  be classified as harmful to Daphnia magna in accordance with the classification system of the EU.

Description of key information

Two studies performed with methods equivalent to EC standards reported EC50 24H on Daphnia magna = 63 mg/L (Palla & Dion, 1983) and EC50 48H = 25.9 mg/l on the same species (Kopperman et al., 1974).

Key value for chemical safety assessment

Additional information

Regarding these 2 studies, guaiacol is considered as harmful for Daphnia.