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Ecotoxicological information

Toxicity to other aquatic organisms

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Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study was not a standardised method but it was well described

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Acute toxicity of substituted phenols to Rana japonica tadpoles and mechanism-based quantitative structure-activity relationship (QSAR) study
Author:
Wang X., Dong Y., Wang L. and Han S.
Year:
2001
Bibliographic source:
Chemosphere, 44 : 447-455
Reference Type:
publication
Title:
Quantitative structure-activity relationships for the toxicity to the tadpole Rana japonica of selected phenols
Author:
Wang X., Dong Y., Xu S., Wang L., and Han S.
Year:
2000
Bibliographic source:
Bull. Environ. Contam. Toxicol., 64 : 859-865

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
evaluate the toxicity on Tadpoles at 12h and 24h.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
It was provided by Department of Chemistry at Nanjing University and was of sufficient purity (analytical purity). Further purification was not necessary.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: All exposures took place at water temperature 22-25°C in a controlled environmental chamber (pH = 6.31). Aerated chloride-free water was used for preparing the stock solutions of chemical and test dilution.
A six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.

Test organisms

Test organisms (species):
Rana sp.
Details on test organisms:
TEST ORGANISM
- Common name: tadpoles
- Strain: Rana Japonica
- Source: collected from a permanent pond in Nanjing.
- Age at study initiation: 30 day-old
- Weight at study initiation: 0.09 +/- 0.01 g
- Length at study initiation: 2.5 +/- 0.1 cm
- Method of breeding: eggs and the resulting tapdoles were cultured in aerated stream water (Cl-: 3.40 mg/L, pH: 7.74, dissolved oxygen concentration: 9.6 mg/L, Na+: 2.35 mg/L; Ca2+: 48 mg/L) in the laboratory at romm temperature (22-25°C).
- Feeding during test
- Food type: an excess of rinsed, salt free canned spinach
- Amount: no data
- Frequency: daily

ACCLIMATION: none

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
12 h
Remarks on exposure duration:
12 and 24 hours

Test conditions

Test temperature:
22-25°C
pH:
measured
Nominal and measured concentrations:
a six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 1000 ml pyrex beaker containing 500 ml test solution or aerated chloride-free water.
- Aeration: no
- Renewal rate of test solution: every 6 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS: aerated chloride-free water

OTHER TEST CONDITIONS:
- Photoperiod: 14 (day) /10 (night) hours.

EFFECT PARAMETERS MEASURED: mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.
- Range finding study: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
12 h
Dose descriptor:
LC50
Effect conc.:
430.44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
392.57 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Reported statistics and error estimates:
Logarithms of inverse of median lethal concentrations after 12-h and 24-h exposure were calculated using non-linear regression in STATISTICA software.

Applicant's summary and conclusion