2-isocyanatoethyl methacrylate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 Dec 1978 - 13 Jul 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Comparable to guideline study with acceptable restrictions: The test with Pimephales promelas was performed at 12 °C. In the current OECD guideline the recommended test temperature range for this species is with 21 - 25 °C much higher. Thus, the low temperature may negatively affect the sensitivity of the test organism by providing unsuitable conditions. However, as no mortality was measured in the control and the observed toxicity in the treatments is rather low the effect of the temperature on the outcome of the study and the aquatic hazard assessment in general can be considered as negligible. The preparation of the test concentrations is not specified. The measurements of the water quality are not stated. No analytical monitoring is performed.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

Test with Pimephales promelas was performed at 12 °C. The recommended test temperature range for this species is given as 21 - 25 °C.

Principles of method if other than guideline:

96 h acute toxicity to fish test conducted similar to current guidelines

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: not specified

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnow
- Source: obtained from Aquatic Systems Inc., Ludington, Michigan, USA on 10 Nov 1978
- Length at study initiation (mean): 2.1 cm
- Weight at study initiation (mean): 0.2 g
- Feeding during test: none

ACCLIMATION
- Acclimation period:Fishes were held in an 800 L fiberglass tub for at least ten days prior to testing.
- Acclimation conditions: Holding conditions were as follows: temperature: 12 °C, photoperiod: 16 h light/ 8 h dark; illumination: 430-646 lux; flow rate: 2 L/min.
- Type and amount of food: synthetic diet
- Feeding frequency: daily
- Health during acclimation: not stated

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

101 mg/L as CaCO3

Test temperature:

12 °C

pH:

7.8

Dissolved oxygen:

not stated

Nominal and measured concentrations:

Nominal: 100, 320, 560 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Fill volume: 10 L
- Aeration: not specified
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Lake Huron water, which had been filtered and dechlorinated through active carbon
- Alkalinity: 88 mg/L as CaCO3
- Conductivity: 192 µmhos/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: not stated

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 1506 - 1722 lux provided by cool white fluorescent bulbs

EFFECT PARAMETERS MEASURED: Mortality was recorded after 24, 48, 72 and 96 h test duration.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

162 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 157 - 168 mg/L

Details on results:

- Mortality of control: No mortality observed.

Reported statistics and error estimates:

The LC50 value was calculated using the moving average method.

Sublethal observations / clinical signs:

Table 1: Mortality of fishes after 96 h test duration

Nominal concentration [mg/L]	Number of exposed fish	Number of dead fishes	Percent dead fishes	Binominal prob (%)
Control	10	0	0	0
100	10	2	20	5.46875
320	10	9	90	1.07422
560	10	10	100	0.0976563
1000	10	10	100	0.0976563

Description of key information

LC50 (96 h) = 162 mg/L (nominal) for Pimephales promelas (equivalent to OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

162 mg/L

Additional information

The acute toxicity of 2 -isocyanatoethyl methacrylate (MOI) to fish was tested in a study conducted by Dow Chemicals (USA, 1979), using fathead minnows (Pimephales promelas) as test species. This static dose-response test (non-GLP) was carried out equivalent to the OECD-Guideline 203 over a period of 96 h. The nominal test concentrations used were 100, 320, 560 and 1000 mg/L. The resulting LC50 was 162 mg/L (nominal).