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EC number: 250-284-7 | CAS number: 30674-80-7
During the third week of exposure, all animals at every dose level and the control group exhibited signs of consistent sialodacryoadentitis (swelling in the neck area and the appearance of a red crusty material around the eyes and nasal area). After approx. ten days, these signs subsided. Beginning with the seventh week of exposure and lasting throughout the rest of the treatment period, five animals at the 250 ppb level exhibited swelling and ulcerated abscesses in the throat region. Control animals appeared normal in appearance and behaviour, and did not show these signs.
Two rats at 250 ppb (high dose group) died during the study. One animal showed signs of a distended and hard abdomen after the first 6 - hour exposure period and died after eleven exposures to 250 ppb of the test material. The second animal died after ten weeks of exposure to 250 ppb of the test item and upon gross examination this animal exhibited a distended and hard abdomen. Gross pathology on both high dose males revealed that death was caused by a large abscessed mass and an infection in the abdominal cavity. Hence, the deaths are considered to be not related to the test material treatment, but attributed to an incidental infection.
Throughout the study, mean body weights of male rats at all dose levels were comparable to those in the control group. Weight losses occurred in some rats during 3rd and 4th weeks of exposure when the signs of sialodacryoadentitis were evident; however, no statistical differences were observed between the rats exposed to the test item and the control animals.
Vaginal plugs were observed with equal frequency beneath the cages of control and of treated rats suggesting that mating occurred in all groups. The fertility indices of male rats exposed to the test item were comparable to the control group. All groups had 95% or higher fertility rate for both weeks of mating. The average number of implantations, corpora lutea, and resorptions in the unexposed female rats bred to the male rats exposed to the test material are given in Tab.1. All exposure levels had values for these parameters which were comparable to the control group. For the first week of breeding the average pre- implantation loss for all dose groups was not significantly different from the control data. For the second week of breeding the average pre- implantation loss at the 80 ppb level was significantly lower than the control mean (Tab. 2). This was not a detrimental effect and was not considered to be of toxicological importance. The average resorption rates in the treatment levels for the first and second week of breeding were comparable to the respective control group resorption rate (Tab. 2).
Tab. 1: Reproductive parameters – average number of implantations, corpora lutea, and resorptions in unexposed female rats bred to male rats exposed to the test item by inhalation
Post exposure breeding
Exposure level [ppb]
Average corpora lutea
13 ± 1
14 ± 1
13 ± 2
1 ± 1
14 ± 2
Mean ± SD. The average number of implantations, corpora lutea, or resorptions for the two females bred to each male was calculated. Group means were then calculated from the averaged values.
No values were significantly different from control values by an analysis of variance and Dunnett’s Test or by a modified Wilcoxon Test, p < 0.05.
Tab. 2: Reproductive parameters – average pre- implantations loss and average resorption rate in unexposed female rats bred to male rats exposed to the test item by inhalation Average pre- implantation loss [%]
Average pre- implantation loss [%]
8 ± 8
6 ± 8
5 ± 5
9 ± 10
7 ± 5
5 ± 6*
9 ± 9
Average resorption rate [%]
5 ± 6
3 ± 5
6 ± 7
5 ± 4
6 ± 4
4 ± 5
* Significantly different from the control mean by a modified Wilcoxon Test, p < 0.05
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