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EC number: 420-640-8 | CAS number: 138495-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Remarks:
- Conducted according to guideline in effect at time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- other: Health Effects Testing Guidelines of US EPA (40 CRF 798.5265)
- Deviations:
- no
- Remarks:
- The study was conducted according to guideline in effect at the time of study conduct.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 420-640-8
- EC Name:
- -
- Cas Number:
- 138495-42-8
- Molecular formula:
- C5H2F10
- IUPAC Name:
- (3R,4R)-1,1,1,2,2,3,4,5,5,5-decafluoropentane; (3S,4S)-1,1,1,2,2,3,4,5,5,5-decafluoropentane
- Details on test material:
- - Purity: 99.68%
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA100, TA1535, TA97, TA98
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9
- Test concentrations with justification for top dose:
- 0, 10, 50, 100, 500, 1000, 2500, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Based on information supplied by the sponsor.
Controls
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO, water
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene(all strains +S9), 2-nitrofluorene (TA98 -S9), sodium azide (TA100 and TA1535 -S9), ICR-191 acridine (TA97 -S9), methyl methane sulfonate (WP2urvA -S9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: The plate incorporation method was applied. Treatment without activation was conducted by adding 0.1 mL of overnight culture containing 1E8 bacteria to top agar supplemented with L-histidine, biotin (for Salmonella strains) or L-tryptophan (for E. coli). The components were mixed and poured onto a plate containing Davis minimal agar. Treatments with activation were conducted as those without activation except that S9 mix was added to the bacteria/top agar mixture before it was poured onto a Davis minimal agar plate. The plates were incubated at approximately 37°C for approximately 48 hours.
DURATION
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 2 trials with 3 treatments per concentration - Evaluation criteria:
- A test substance was classified as positive when (1) the average number of revertants in any strain at any test substance concentration studied is at least 2 times greater than the average number of revertants in the negative control AND (2) there is a positive dose-response relationship in that same strain. A test substance is classified as negative when (1) there are no test substance concentrations with an average number of revertants which is at least 2 times greater than the average number of revertants in the solvent control OR (2) there is no positive dose-response relationship. A test substance is classified as equivocal when the test substance is not clearly negative yet does not meet the criteria for a positive response.
- Statistics:
- Trials were evaluated independently. For each selected tester strain, the average number of revertants and the standard deviation at each concentration with and without S9 activation were calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA100, TA1535, TA97, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY: Background lawn was noticeably thinner and/or size of microcolonies slightly larger than controls in TA1535 at 2500 µg/plate (without activation) and TA98 at 5000 µg/plate (without activation). Background lawn was markedly thinner and/or microcolonies were markedly larger than controls in TA1535 at 5000 µg/plate(without activation).
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability). The test substance was negative when tested in Salmonella typhimurium strains TA100, TA1535, TA97, and TA98 and in Escherichia coli WP2uvrA (pKM101) in the absence and presence of S9 activation.
- Executive summary:
The test substance was evaluated for mutagenicity in Salmonella typhimurium strains TA100, TA1535, TA97, and TA98 and in Escherichia coli WP2uvrA (pKM101) with and without an exogenous metabolic activation system (S9). The maximum concentration tested was 5000 µg/plate. No evidence of mutagenic activity was detected in either of two independent trials. In this study, the test substance was negative.
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