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EC number: 420-640-8 | CAS number: 138495-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- Remarks:
- Study conducted according to guideline in effect at time of study conduct
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 420-640-8
- EC Name:
- -
- Cas Number:
- 138495-42-8
- Molecular formula:
- C5H2F10
- IUPAC Name:
- (3R,4R)-1,1,1,2,2,3,4,5,5,5-decafluoropentane; (3S,4S)-1,1,1,2,2,3,4,5,5,5-decafluoropentane
- Details on test material:
- - Purity: 99%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations Sampled: (measured values) 0, 0.648, 1.72, 4.19, 9.1, 21.8 mg/L; (nominal values) 0, 1.28, 3.20, 8.00, 20.0, 50.0 mg/L)
- Sampling Times: Fresh media (0-hour): Days 0, 1, 5, 6, 12, 19 and Old media (48-hours) Days: 1, 2, 6, 7, 8, 13, 20
- Sample storage conditions before analysis: Stored at 6 ± 2ºC
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION: The stock solution (100 mg/L) was prepared with dilution water one day prior to application. For preparation of the stock solution 63.3 µL/L of the test substance was added below the water surface. The stock solution was shaken with an overhead shaker adjusted to 20 rpm for 24 hours at room temperature. Test concentrations were achieved by serial dilution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia (water flea)
- Strain/clone: STRAUS/Clone 5
- Feeding during test: At least 5 times per week. For each test vessel Pseudokirchneriella subcapitata (0.470 - 0.765 mL) and Desmodesmus subspicatus (0.890 - 1.05 mL) suspensions were added as food.
ACCLIMATION
- Acclimation period: not reported
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- none
Test conditions
- Hardness:
- Hardness ranged from 165 - 178 mg CaCO3
- Test temperature:
- Temperature ranged from 20.9 - 22.0 °C
- pH:
- pH ranged from 7.78 - 8.97
- Dissolved oxygen:
- Dissolved oxygen ranged from 8.19 - 9.87 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 1.28, 3.20, 8.00, 20.0, and 50.0 mg/L
Measured: 0, 0.648, 1.72, 4.19, 9.10 and 21.8 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Sealed glass flask
- Renewal rate of test solution (frequency/flow rate): Renewed daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
OTHER TEST CONDITIONS
- Photoperiod: 16/8 light/dark cycle
- Light intensity: Maximum 20 µE me-2 se-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Mortality of parent Daphnia, daily
- First appearance of juveniles (neonates), daily
- Condition of parent Daphnia (swimming behavoir, number of males, winter eggs, other abnormalities), daily
- Total length and dry body weight of parent Daphnia, daily
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0, 1, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- >= 6.17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Parental survival
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- >= 4.19 - < 9.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Offspring survival
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.72 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Offspring survival
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- >= 4.19 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Offspring survival
- Details on results:
- Mortality of parent animals: At concentrations of 9.10 and 21.8 mg/L mortality was 100%
The mean dry weght of surviving parent animals decreased with increasing concentration of test substance. For samples 0, 0.648, 1.72, and 4.19 mg/L the respective weights were 1.31, 1.13, 0.97, and 0.89 mg. - Results with reference substance (positive control):
- Reference substance: Potassium dichromate
Test concentrations: 0.625, 1.00, 1.60, and 2.56 mg/L
Range of validity: 1.0 mg/L < EC50 (24 h) < 2.5 mg/L, according to AQS P 9/2 (clone 5)
Range of validity: 0.6 mg/L < EC50 (24 h) < 2.1 mg/L, according to OECD 202 (clone A)
Test duration: 24 hours - Reported statistics and error estimates:
- The NOEC and LOEC for reproduction were determined directly from the results. Significant deviations were determined in comparison to the control group using statistical standard procedures as Normality Test, Equal Variance Test and Analysis of Variance. The EC50 value of the adult mortality was determined by calculating the geometric mean of the highest concentration causing no adult mortality and the lowest concentration causing 100% adult mortality, because standard methods of calculating the EC50 value were not applicable to the data obtained.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
EC50 adult mortality = 6.17 mg/L
EC50 reproduction ≥4.19, but <9.1 mg/L
NOEC reproduction = 1.72 mg/L
LOEC reproduction = 4.19 mg/L - Executive summary:
A semi-static, 21-day reproduction test in Daphnia magna was performed at measured concentrations of 0, 0.648, 1.72, 4.19, 9.1, and 21.8 mg/L. The average number of juveniles per parent in the control group was 98 after 21 days. The reproductive output was statistically significantly reduced (by 28.6%) only at the mean measured concentration of 4.19 mg/L when compared to control. No juveniles were produced at concentrations of 9.10 and 21.8 mg/L as adult mortality was 100%. The adult mortality was 100% at the mean measured concentrations of 9.10 and 21.8 mg/L after 21 days. The test substance did not induce significant mortality (>20%) of parental animals at any of the other concentrations and in the control. At the control as well as mean measured concentrations 0.648 and 4.19 mg/L all daphnids survived until the end of the study. The EC50 for adult mortality after 21 days was calculated to be 6.17 mg/L.
The Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) based on reproductive output were, respectively, 4.19 and 1.72 mg/L.
Related to the total number of produced juveniles (dead + alive) the percentage of stillborn juveniles and aborted eggs was ≤4% at the mean measured concentrations 0.648, 1.72 mg/L, and control. Only at the mean measured concentration of 4.19 mg/L was there a noticeable increase (13%) in stillborn juveniles and aborted eggs.
No males and ephippia (winter eggs) occurred in control or test groups.
The mean day of release of the first brood was day 8.4 at the control group. At the mean measured concentrations of 0.648 and 4.19 mg/L the mean day of release of the first brood was in the range of 8.5 to 8.9. The first brood was released until day 9 by all daphnids of the control group and the test concentration levels except one parent animal each at concentrations 0.648 and 1.72 mg/L. Four broods were released by all surviving animals of the test and control groups.
The dry body weight and total length of the parent daphnids in the test concentrations were comparable to the control group. The mean dry body weight at the tested levels ranged from 0.89 to 1.13 mg per daphnid. The mean dry body weight at the control was 1.31 mg per daphnid. The mean total body length for control daphnids was 5.40 mm and, for tested concentrations, in the range of 4.85 to 5.40 mm.
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