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EC number: 420-640-8
CAS number: 138495-42-8
semi-static, 21-day reproduction test in Daphnia magna was performed at
measured concentrations of 0, 0.648, 1.72, 4.19, 9.1, and 21.8 mg/L. The
average number of juveniles per parent in the control group was 98 after
21 days. The
reproductive output was statistically significantly reduced (by 28.6%)
only at the mean measured concentration of 4.19 mg/L when compared to
juveniles were produced at concentrations of 9.10 and 21.8 mg/L as adult
mortality was 100%. The
adult mortality was 100% at the mean measured concentrations of 9.10 and
21.8 mg/L after 21 days. The
test substance did not induce significant mortality (>20%) of parental
animals at any of the other concentrations and in the control. At
the control as well as mean measured concentrations 0.648 and 4.19 mg/L
all daphnids survived until the end of the study. The
EC50 for adult mortality after 21 days was calculated to be 6.17 mg/L.
Lowest Observed Effect Concentration (LOEC) and the No Observed Effect
Concentration (NOEC) based on reproductive output were, respectively,
4.19 and 1.72 mg/L.
to the total number of produced juveniles (dead + alive) the percentage
of stillborn juveniles and aborted eggs was ≤4% at the mean measured
concentrations 0.648, 1.72 mg/L, and control. Only
at the mean measured concentration of 4.19 mg/L was there a noticeable
increase (13%) in stillborn juveniles and aborted eggs.
males and ephippia (winter eggs) occurred in control or test groups.
mean day of release of the first brood was day 8.4 at the control group. At
the mean measured concentrations of 0.648 and 4.19 mg/L the mean day of
release of the first brood was in the range of 8.5 to 8.9. The
first brood was released until day 9 by all daphnids of the control
group and the test concentration levels except one parent animal each at
concentrations 0.648 and 1.72 mg/L. Four
broods were released by all surviving animals of the test and control
dry body weight and total length of the parent daphnids in the test
concentrations were comparable to the control group. The
mean dry body weight at the tested levels ranged from 0.89 to 1.13 mg
per daphnid. The
mean dry body weight at the control was 1.31 mg per daphnid. The
mean total body length for control daphnids was 5.40 mm and, for tested
concentrations, in the range of 4.85 to 5.40 mm.
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