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EC number: 420-640-8 | CAS number: 138495-42-8
Initial Measured Concentration (mg/L)
The test was performed under static conditions with 6 concentrations (0, 11, 18, 31, 50, 84, and 140 mg/L) of test substance and a dilution water control at 22.5±1.5°C. The test substance was formulated into a supersaturated stock solution (1000 mg/L) in dilution water, mixed in a sealed container for 24 hours at 30°C, and allowed to equilibrate to test temperature for 24 hours. Water was carefully removed from the mixing vessel to avoid insoluble material and used immediately to initiate the toxicity test. Because the stock solution contained a greater concentration of test substance than the mean solubility limit of 116 mg/L (probably as the result of the accidental inclusion of undissolved test substance), nominal concentrations may be artificially high. Initial measured concentrations were ND, 9.26, 14.1, 22.9, 36.8, 60.7, and 108 mg/L. Because of the volatile nature of the test substance, the test was performed in sealed 3.8 L test vessels that contained 3 L of test solution. Vessels were not opened during the exposure period unless complete mortality occurred. All animals were in good condition at the beginning of the study and 100% of the control fish survived the toxicity test. Exposure of fathead minnows to the test substance resulted in a 96-hour LC50 of 27.2 mg/L, with a 95% confidence interval of 22.9-36.8 mg/L. The 96-hour NOEC was 14.1 mg/L
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