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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The LC50 after 96 hours was 14.1 (10.0 - 20.0) mg/L (nominal test item concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
14.1 mg/L

Additional information

The acute toxicity of the test item to zebrafish was determined according to the principles of the OECD-Guideline No. 203 and EU Method C.1. It is therefore, given the reliability rating of 1 (reliable without restrictions).

An acute toxicity test under semi-static conditions with renewal of the test media after 48 hours was conducted with the nominal test item concentrations of 1.25, 2.50, 5.00, 10.0 and 20.0 mg/L (factor 2). Duration of the test was 96 hours. Seven test organisms were exposed to each test concentration and the control. Water quality parameters (i.e. temperature, pH-value and oxygen- saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits.

All validity criteria of the test guideline were met.

The test item was found to cause lethal effects to zebrafish after 96 hours at a nominal test item concentration of 20.0 mg/L. The LC0 and LC100 after 96 hours was 10.0 mg/L and 20.0 mg/L (nominal test item concentration). The LC50 after 96 hours was 14.1 (10.0 - 20.0) mg/L (nominal test item concentration).