Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline modified.

Data source

Reference
Reference Type:
publication
Title:
A Comparison of the Toxicity of 50 Reference Chemicals to Freshly Isolated Rainbow Trout Hepatocytes and Daphnia magna
Author:
Lilius H, Isomaa B, Holmstrom T
Year:
1994
Bibliographic source:
Aquatic Toxicology 30:47-60

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
Not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Warfarin.

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
Warfarin first disolved in methanol.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna.
- Source: Department of Biology, University of Turku.
- Age at study initiation (mean and range, SD): < 24 hours.
- Feeding during test
- Frequency: Not fed during test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
None.

Test conditions

Hardness:
Not specified.
Test temperature:
21 ± 1° Celcius.
pH:
7.6 SU.
Dissolved oxygen:
Not specified.
Salinity:
Not applicable.
Nominal and measured concentrations:
5 concentrations, not specified.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Closed.
- Material, size, headspace, fill volume: 100 ml polystyrene, 50 ml test solution volume.
- Aeration: No.
- No. of organisms per vessel: 20.
- No. of vessels per concentration (replicates): 3.
- Culture medium different from test medium: Yes.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light, 12 hours dark.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations made for immobilization at 24 hours.


Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
0.288 other: mM
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: ± 0.007 mM
Reported statistics and error estimates:
The GraphPad 4 statistical program was used to calculate the LC50.

Applicant's summary and conclusion

Conclusions:
The 24 hour LC50 for Warfarin to Daphnia magna was found to be 0.288 ± 0.007 mM.