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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Comparative Acute Toxicity of the First 50 Multicentre Evaluation of In Vitro Cytotoxicity Chemicals to Aquatic Non-Vertebrates
Author:
Calleja MC, Persoone G, Geladi P
Year:
1994
Bibliographic source:
Archives of Environmental Contamination and Toxicology, 26, 69-78

Materials and methods

Test guideline
Qualifier:
no guideline available
Guideline:
other: manufacturer's SOP
Deviations:
no
Remarks:
- Performed to standard assay procedures (Microbics 1989.)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Warfarin.
- Analytical purity: >97 %.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Photobacterium phosphoreum
Details on inoculum:
Not specifed.

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
5 min
Remarks on exposure duration:
5-15 minutes
Post exposure observation period:
None.

Test conditions

Hardness:
Not specified.
Test temperature:
Not specified.
pH:
Not specified.
Dissolved oxygen:
Not specified.
Salinity:
Not specified.
Nominal and measured concentrations:
Not specified.
Details on test conditions:
Not specified.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
5 min
Dose descriptor:
EC50
Effect conc.:
219 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: 95 % CI not given
Details on results:
Not specified.
Reported statistics and error estimates:
EC50 obtained by linear regression package provided by the Microbics Corporation (manufacturer of the Microtox product).

Any other information on results incl. tables

The 5 and 15 minute EC50s of the Microtox test were obtained by the linear regression package provided by the Microbics Corportation.

Applicant's summary and conclusion

Conclusions:
The reported EC50 was 219 µmol/L on Photobacterium phosphoreum using the Microtox(TM) test.