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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not GLP.

Data source

Reference
Reference Type:
publication
Title:
The Acute Toxicity of 102 Pesticides and Miscellaneous Substances to Fish
Author:
Tooby TE, Hursey PA, Alabaster JS
Year:
1975
Bibliographic source:
Chemistry and Industry, 21: 523-526

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Alabaster and Abram 1965a
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Warfarin.
- Analytical purity: Commercial formulation, 100%.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Rasbora heteromorpha
Details on test organisms:
TEST ORGANISM
- Common name: Harlequin fish.
- Strain: Duncker.
- Length at study initiation (length definition, mean, range and SD): 1-3 cm.


ACCLIMATION
- Acclimation period: At least a week.
- Acclimation conditions (same as test or not): Yes.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None.

Test conditions

Hardness:
20 mg/L as CaCO3.
Test temperature:
20° Celcius.
pH:
8.1 SU.
Dissolved oxygen:
Not specified.
Salinity:
Not applicable.
Nominal and measured concentrations:
Not specified.
Details on test conditions:
TEST SYSTEM
- Test vessel: Not specified.
- Aeration: No.
- Renewal rate of test solution (frequency/flow rate): Daily.


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Not specified.


OTHER TEST CONDITIONS
- Adjustment of pH: No.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48
Dose descriptor:
LC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Reported statistics and error estimates:
LC50s calculated after Litchfield and Wilcoxon, 1949.

Applicant's summary and conclusion

Conclusions:
The 96 hour LC50 for Warfarin to Harlequin fish is 12 mg/L.