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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Deion. water instead of dest. water for the stock solution. 4 instead of 5 test concentration. Preparation of he inoculum: Higher sludge weight after the washing procedure (sludge concentrations in the test according to the Guidelines).
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Test substance:
Code number: FAT-40'061/D
EN-number: 280736.26
Purity: 98.3
Appearance: Solid
Solubility: 100 g/l in water
Storage: room temperature
Expiration date: may 1998
Safety: Test substance will, unless otherwise specified, be handled using safety precautions "Sicherheit im Labor von A - Z", SAV: 00 303
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel).
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20.2 °C
pH:
pH of sludge: 6.6 before the test beginning.
Dissolved oxygen:
0.1 - 0.2 l/min by pasteur-pipette
Nominal and measured concentrations:
Test concentrations:
320, 100, 32 and 10 mg/l of the test substance

32, 10 and 3.2 mg/l of the reference substance.
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol (Merck 821768)
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The IC50 (3 h) of the test substance FAT 40061/D : >320.0 mg/l.
Results with reference substance (positive control):
The IC50 (3 h) of the reference substance 3,5-Dichlorophenol : 7.8 mg/l.
Validity criteria fulfilled:
yes
Conclusions:
The IC50 (3 h) of the test substance FAT 40061/D: >320.0 mg/l.
Executive summary:

The objective of this study was the determination of the test substance concentration, which reduces the respiration of activated sludge by 50 % (IC50 value). The toxicity of FAT 40061/D to activated sludge was determined in a 3-hour respiration inhibition test according to the OECD Guideline No. 209, and to the EG Method Nr.L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance concentrations tested were 320,100, 32 and 10 mg/l and the % inhibitions obtained were 0, 0, 0 and 4.44 % respectively. Based on these findings, IC50 (3 h) of the test substance FAT 40061/D was determined to be >320.0 mg/l.

Description of key information

The objective of this study was the determination of the test substance concentration, which reduces the respiration of activated sludge by 50 % (IC50 value). The toxicity of FAT 40061/D to activated sludge was determined in a 3 hours respiration inhibition test according to the OECD Guideline No. 209, and to the EG Method Nr.L 133/118. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance concentrations tested were 320,100, 32 and 10 mg/l and the % inhibitions obtained were 0, 0, 0 and 4.44 % respectively. Based on these findings,IC50 (3 h) of the test substance FAT 40061/D was determined to be >320.0 mg/l. In the other supporting study, the IC50 value for FAT 40061/A was found to be greater than 300 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
320 mg/L

Additional information