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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information


The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave the following results:
The EC50 (growth rate) was > 100 mg/L.
The NOEC was 32 mg/L and the LOEC was 100 mg/L.
The EC50 (yield) was > 100 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
32 mg/L

Additional information

In a GLP compliant algal growth inhibition test was performed in accordance to OECD guideline 201.

Pseudokirchneriella subcapitata strain was exposed to FAT 40061/F at different concentrations 0.10, 1.0, 10.0 and 100mg/L for aperoid of 72 hrs. Since the test item (FAT 40061) formed colored test solutions, spectrophotometer readings were taken at both 460 and 665 nm inorder to determine whether significant light absorption occurred.

After 72 hrs, cell density of each flask was determined. The average specific growth rate over the test duration was calculated for each replicate control and test item vessel using nominally inoculated cell concentration as th starting value rather than the measured starting value in order to increase the precision of the calculation.

Percentage inhibition of growth rate and yield were calculated for each replicates.

The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave the following results:

The EC50 (growth rate) was > 100 mg/L.

The NOEC was 32 mg/L and the LOEC was 100 mg/L.

The EC50 (yield) was > 100 mg/L.