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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1974
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Test organisms (species):
other: Rainbow trout
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen

Further standard conditions:
- Basins have been cleaned with RBS-35 solution (FLUKA 83462) and rinsed with clean water.
- Testing water should be kept at a pH between 6 and 8.5. In case of deviations, pH needs to be adjusted with NaOH or HCl solution.
- Acclimatization period: minimum 12 days at 10-13 °C for trout.
- Feeding periods: daily (TETRA MIN, flocks, Dr. U.Baersch GmbH, D-4520 Melle, Germany)
- Day-night cycles: 12/12h
- Termination: For termination of the experiment MS-222 (Sandoz) should be used at 1 % (Methacain).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
14 °C
pH:
7.4-8.1
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 100, 200 and 1000 mg/L
Details on test conditions:
Water:
- 15 liters for trout, Water used according to ISO guidelines.

Basins:
- Full-glass basins, 17 liters basis (36x23x26 cm) tempered (cold-room); 5 liter basins (15x20x20 cm) tempered
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
570 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality was seen at 100 and 200 mg/L concentrations, however at 1000 mg/L, 6 out of 8 fish were found to have died during the 48 hour observation period.
Validity criteria fulfilled:
yes
Conclusions:
The TL50 of FAT 40061/A in this fish acute toxicity test was determined to be 570 mg/L.
Executive summary:

The acute fish toxicity test was performed using a methodology equivalent or similar to OECD Guideline 203. Rainbow trout were used in this test. Groups of fish, each containing 8 fish, were exposed to test concentrations of 100, 200 and 1000 mg/L. No mortality was seen at 100 and 200 mg/L concentrations, however at 1000 mg/L, 6 out of 8 fish were found to have died during the 48 hour observation period. Based on these findings, the 48 h TL50 of FAT 40061/A was determined to be 570 mg/L.

Description of key information

The acute fish toxicity test was performed using a method equivalent or similar to OECD Guideline 203. Rainbow trout were used in this test. Groups of fish, each containing 8 fish, were exposed to test concentrations of 100, 200 and 1000 mg/L.No mortality was seen at 100 and 200 mg/L concentrations, however, at 1000 mg/L, 6 out of 8 fish were found to have died during the 48-hour observation period. Based on these findings, the 48 h TL50 of FAT 40061/A was determined to be 570 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
570 mg/L

Additional information