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The toxicity of Reactive Yellow 039 was assessed on all three trophic levels of aquatic ecosystem. The TL50 of FAT 40061/A in the fish acute toxicity test was determined to be 570 mg/L.The EC50 and EC100 values were clearly higher than 189.0 mg/l when FAT 40061/A was exposed to Daphnia. The exposure of FAT 40061/F to algae resulted in te EC50 (growth rate) >100 mg/L and the NOEC being 32 mg/L. Additionally, Reactive Yellow 039 was found to have the IC50 greater than 320 mg/L when exposed to microbial population.

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