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An acute fish toxicity test was performed according to OECD guideline 203.

Rainbow trout were used in this test. Fishes were tested at these following different concentrations: 100 / 200 and 1000 mg/L

- At 100 mg/L: all 8 fishes where alive at 24 and 48 hours of exposure time (pH was 7.4 after 48 hours exposure time).

- At 200 mg/L: all 8 fishes where alive at 24 and 48 hours of exposure time (pH was 8.1 after 48 hours exposure time).

- At 1000 mg/L: 3 fished survived after 24 hours exposure time and 2 after 48 hours (pH was 7.6 after 48 hours exposure time)

The TL50 of FAT 40061/A in this fish acute toxicity test was determined to be 570 mg/L.

The acute toxicity of FAT 40061/D to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2.

The test was performed in compliance with the Good Laboratory Practice Regulations.

The nominal concentrations tested were 18, 32, 58, 100 and 180 mg test article/l, and a control without any additions.

During test duration the test article concentrations in the analyzed test media were in the range of 103.7 - 107.2 of the nominal values. Therefore, under test conditions the test article was sufficiently stable.

In all test concentrations no alteration of the test substance after 48 hours was observed. The reported results are related to the analytical concentrations of the test article.

In the control and in all test concentrations no immobilization of the daphnias’ was observed after 48 hours. The EC0 value is at least 189.0 mg /l, but might even be higher than this concentration. The EC50 and EC100 values are clearly higher than 189.0 mg /l.

Therefore, the EC50 value could not be calculated.


A GLP compliant algal growth inhibition test was performed in accordance to OECD guideline 201.

Pseudokirchneriella subcapitata strain was exposed to FAT 40061/F at different concentrations 0.10, 1.0, 10.0 and 100mg/L for a period of 72 hrs. Since the test item (FAT 40061) formed colored test solutions, spectrophotometer readings were taken at both 460 and 665 nm in order to determine whether significant light absorption occurred.

After 72 h, cell density of each flask was determined. The average specific growth rate over the test duration was calculated for each replicate control and test item vessel using nominally inoculated cell concentration as the starting value rather than the measured starting value in order to increase the precision of the calculation. Percentage inhibition of growth rate and yield were calculated for each replicates.

The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave the following results:

The EC50 (growth rate) was > 100 mg/L.The NOEC was 32 mg/L and the LOEC was 100 mg/L.The EC50 (yield) was > 100 mg/L.

A study was carried out for bacterial growth inhibition where an inhibition concentration (IC50) of FAT 40061/A was determined.

Activated sludge was exposed to FAT 40061/A to assess the toxicity to microorganisms. About 100 ml of the underrun of a STP is taken and filtered through a sterile filter. Subsequently this filtrate is used to prepare the following dilutions. The IC50 value for FAT 40061/A was found to be greater than 300 mg/L.

In the other supporting study, the IC50 value for FAT 40061/D was found to be greater than 320 mg/L.