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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The inherent and ready biodegradability of FAT 40061 was determined according to OECD guidelines.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A key study was performed on the test substance FAT 40061/D according to DEV H 41-1, DEV 1980. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

 The test item was dissolved in water, further oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test substance concentration was tested in 5.0 mg/l. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/l.

The COD of FAT 40061/D was found to be 800 mg O2/g, whereas the COD of the reference substance was 197 mg O2/l.

 

In the other study the biochemical oxygen demand of FAT 40061/D was determined according to ISO 5815 Second Edition 1989-08-01 (E). The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

The BOD was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water, saturated with dissolved oxygen and containing a seed of microorganisms. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance being D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/l.

The BOD of FAT 40061/D corrected by the blank control is: 6 mg O2/g, whereas the BOD of glucose/glutamic acid (reference substance) solution is: 192 mg O2/I.

 

In the other key study, The inherent biodegradability of FAT 40061/D was determined in a 28 days (Zahn-Wellens – Test) according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.

The test substance was tested in concentration of 150 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC.

The bioelimination of the test substance FAT 40061/D after 28 days is: 0 %, whereas the bioelimination of the reference substance after 19 days is: 90.8 %. Therefore, the test item FAT 40061/D is considered as non-biodegradable.

 

In the supporting studies, the Chemical Oxygen Demand of FAT 40061/A was determined to be 789.91 mg/g O2, whereas the Biological Oxygen Demand of FAT 40061/A was determined to be 56 mg/g O2 at 50 mg/l concentration.