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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods
Qualifier:
according to
Guideline:
ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
Deviations:
yes
Remarks:
Aerate over night after addition of the inoculum.
GLP compliance:
yes
Specific details on test material used for the study:
Test substance
The test substance is identified by the Code number. Selection, identification and characterization is guaranteed by the sponsor
Code number: FAT-40'061/D
EN number: 280736.26
Purity: 98.3 %
Appearance: Solid
Solubility: 100 g/l (in water)
Storage: room temperature
Expiration date: May 1998
Safety: Test substance will, unless otherwise specified, be handled using safety precautions "Sicherheit im Labor von A - Z", SAV: 00 303
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
None
Duration of test (contact time):
5 d
Initial conc.:
825.2 mg/L
Based on:
test mat.
Initial conc.:
412.6 mg/L
Based on:
test mat.
Initial conc.:
206.3 mg/L
Based on:
test mat.
Initial conc.:
103.2 mg/L
Based on:
test mat.
Initial conc.:
51.6 mg/L
Based on:
test mat.
Initial conc.:
25.8 mg/L
Based on:
test mat.
Initial conc.:
12.9 mg/L
Based on:
test mat.
Initial conc.:
6.4 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
other: D(+)-Glucose and L-Glutamic acid
Test performance:
None
Remarks on result:
not measured/tested
Details on results:
None
Parameter:
BOD5
Value:
6 mg O2/g test mat.
Results with reference substance:
The BOD5 of glucose/glutamic acid solution was 192 mg O2/I.
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The BOD of FAT 40061/D corrected by the blank control is: 6 mg 02/g.
Executive summary:

The Biological Oxygen Demand (BOD5) of FAT 40061/D was determined according to according to ISO 5815 Second Edition 1989-08-01 (E). This is achieved by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test substance was tested in concentrations from about 6 to 800 mg/l. Based on these investigations, the BOD5 of test substance was found to be 6 mg O2/g. Considering the COD value determined with the test substance which is 800 mg O2/g, the resulting BOD5/COD quotient is 0.0075, indicating that the test substance is unlikely to be biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
other: EEC Directive 84/449 (1984), Part. C.9
Deviations:
yes
Remarks:
Mean of 2 determinations instead of 3.
Qualifier:
according to
Guideline:
other: DEV H 41-1, Deutsches Einheits-Verfahren, 1980
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
Test substance
The test substance is identified by the Code number. Selection, identification and characterization is
guaranteed by the sponsor
Code number: FAT-40*061/D
EN number: 280736.26
Purity: 98.3 %
Appearance: Solid
Solubility: 100 g/l (in water)
Storage: room temperature
Expiration date: May 1998
Safety: Test substance will, unless otherwise specified, be handled using safety precautions "Sicherheit im Labor von A - Z", SAV: 00 303
Oxygen conditions:
other: not required as COD was determined
Inoculum or test system:
other: not required as COD was determined
Duration of test (contact time):
2 h
Reference substance:
other: Potassium hydrogen phtalate
Remarks on result:
not measured/tested
Details on results:
None
Parameter:
COD
Value:
800 mg O2/g test mat.
Results with reference substance:
The COD of the reference substance was 197 mg O2/I (Criteria: 200 ± 8 mg O2/I).
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The COD of FAT 40061/D was determined to be 800 mg O2/g
Executive summary:

The COD of FAT 40061/D was determined in a study performed in accordance with EEC Directive 84/449 (1984), Part. C.9 according to DEV H 41-1, Deutsches Einheits-Verfahren, 1980. 5 mg FAT - 40061/D dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The COD of the test substance was calculated from the amount of unreacted dichromate, determined by titration with standardized ferrous ammonium sulphate. Using the methodology, the COD of FAT 40061/D was found to be 800 mg O2/g.

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: ISO Method 8192 B
Deviations:
no
Qualifier:
according to
Guideline:
other: EEC Directive, L133 (pp. 99-105) Volume 31, May 88 (ISSN 0378-6978)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Test substance:
Code number: FAT - 40'061/D
EN number: 280736.26
Purity: 98.3%
Appearance: Solid
Solubility: 100 g/l (in water)
Storage: room temperature
Expiration date: May 1998
Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Details on inoculum:
None
Duration of test (contact time):
28 d
Initial conc.:
150 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Reference substance:
diethylene glycol
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Details on results:
Test substance:
The bioelimination of FAT 40061/D measured as DOC after 28 days corrected by the blank control is: 0 % (average of 2 tests running in parallel)
Adsorption after 3 hours: 3.9 %
Results with reference substance:
The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 90.8 % on 19th day.
Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
The bioelimination of FAT 40061/D measured as DOC after 28 days corrected by the blank control was 0 %.
Executive summary:

The inherent biodegradability of FAT 40061/D was determined in a 28 days (Zahn-Wellens – Test) according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC. Sludge of a biological sewage treatment plant (ARA-Basel Industrie) collected on 13.04.93 was used as test organism. After 28 days exposure at 22.0 ± 3°C, the DOC was estimated using Shimadzu analyzer. The bioelimination was 0 % when measured as DOC corrected by the blank control.

Description of key information

Reactive Yellow 039 was found to be neither readily nor inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information

The BOD5 of FAT 40061/D was found to be 6 mg O2/g, while the COD was 800 mg O2/g. The resulting BOD5/COD quotient is 0.0075, indicating that FAT 40061/D is not rapidly biodegradable. The inherent biodegradability of FAT 40061/D was determined in a 28 days (Zahn-Wellens – Test) according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The bioelimination of the test substance FAT 40061/D after 28 days was 0 %. Therefore, the test item FAT 40061/D is considered as not inherently biodegradable. In the supporting studies, the Chemical Oxygen Demand of FAT 40061/A was determined to be 789.91 mg/g O2, whereas the Biological Oxygen Demand was determined to be 56 mg/g O2 at 50 mg/l concentration. Thus BOD5/COD ratio was <0.5, indicating FAT 40061/A to be not rapidly biodegradable.