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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well-documented, peer-reviewed publication.

Data source

Reference
Reference Type:
publication
Title:
Risk assessment of linear alkylbenzene sulphonates, LAS, in agricultural soil revisited: Robust chronic toxicity tests for Olsomia candida (Collembola), Aporrectodea caligninosa (Oligochaeta), and Enchytraeus (Enchytraeidae)
Author:
Krogh, PH, Lopez, CV, Cassani, G, Jensen, J, Holmstrup, M, Schraepen, N, Jorgensen, E, Gavor, Z, Temara, A
Year:
2007
Bibliographic source:
Chemosphere 69, 872-879.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
GLP compliance:
no
Application method:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Petresul 550
- Physical state: aqueous solution
- Analytical purity: 47.2 active ingredient
- Composition of test material, percentage of components: C10 14.2%, C11 35.6%, C12 29.6%, C13 20.6%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Initial concentrations were measured.

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
LAS was mixed in demineralized water, and then added to dry Askov soil. Fresh sludge (approx. 200 g) was freeze-dried. This was crushed and sieved through a 2 mm sieve. A methanol solution of LAS was then added to the sludge.

Test organisms

Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)
Details on test organisms:
TEST ORGANISM
- Source: permanent laboratory cultures
- Age at test initiation (mean and range, SD): 10-12 days

Study design

Study type:
laboratory study
Total exposure duration:
28 d

Test conditions

Details on test conditions:
TEST SYSTEM
- Amount of soil or substrate: 26 g soil mixed with 4 ml test solution
- No. of replicates per treatment group: 3

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 76
- % silt: 12
- % clay: 11
- Soil classification system: sandy loam
- Organic carbon (%):1.3
- CEC: 13.4

Nominal and measured concentrations:
25-300 mg/kg soil

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
205 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
reproduction
Details on results:
Reproduction was not significantly affected, but an indicative EC10 was estimated at 205 mg/kg soil.

Applicant's summary and conclusion

Conclusions:
The EC10 for Folsomia candida based on reproduction was 205 mg/kg soil.
Executive summary:

Groups of F. candida were exposed to concentrations of 25-300 mg LAS/kg soil. Exposure lasted 28 days, after which adult survival and reproductive parameters were examined. The EC10 based on reproduction was 205 mg/kg soil.