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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well-documented, peer-reviewed publication.

Data source

Reference
Reference Type:
publication
Title:
Risk assessment of linear alkylbenzene sulphonates, LAS, in agricultural soil revisited: Robust chronic toxicity tests for Olsomia candida (Collembola), Aporrectodea caligninosa (Oligochaeta), and Enchytraeus (Enchytraeidae)
Author:
Krogh, PH, Lopez, CV, Cassani, G, Jensen, J, Holmstrup, M, Schraepen, N, Jorgensen, E, Gavor, Z, Temara, A
Year:
2007
Bibliographic source:
Chemosphere 69, 872-879.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Similar to ISO (1998) and ISO 16387 (2004).
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Petresul 550
- Physical state: aqueous solution
- Analytical purity: 47.2 active ingredient
- Composition of test material, percentage of components: C10 14.2%, C11 35.6%, C12 29.6%, C13 20.6%

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Initial concentrations were measured.

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
LAS was mixed in demineralized water, and then added to dry Askov soil. Fresh sludge (approx. 200 g) was freeze-dried. This was crushed and sieved through a 2 mm sieve. A methanol solution of LAS was then added to the sludge.

Test organisms

Test organisms (species):
other: A. caliginosa, E. crypticus
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: surrounding of National Environmental Research Institute, Silkeborg, Denmark

ACCLIMATION
- Acclimation period: 7 days

TEST ORGANISM
- Source: permanent laboratory cultures

Study design

Study type:
laboratory study
Substrate type:
natural soil
Total exposure duration:
28 d

Test conditions

Test temperature:
16 degrees C
Details on test conditions:
A. caliginosa
TEST SYSTEM
- Test container (material, size): plastic pots
- Amount of soil or substrate: 1 kg soil and 80 ml test solution
- No. of organisms per container (treatment): 4
- No. of replicates per treatment group: 6
- No. of replicates per control: 2

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 76
- % silt: 12
- % clay: 11
- Soil classification system: sandy loam
- Organic carbon (%):1.3
- CEC: 13.4


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cocoons were collected by sieving with 1 mm mesh after 28 days.



E. crypticus

- Amount of soil or substrate: 20 g soil and 3 ml test solution
- No. of organisms per container (treatment): 3

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 76
- % silt: 12
- % clay: 11
- Soil classification system: sandy loam
- Organic carbon (%):1.3
- CEC: 13.4
Nominal and measured concentrations:
A. caliginosa: 25-150 mg/kg soil (nominal)
E. crypticus 0, 10, 40, 60, 80, 100, 120 mg/kg soil (nominal)
0.65, 7.1, 26.3, 45.5, 60.3, 92.4, 102.7 mg/kg soil (measured)

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
EC10
Effect conc.:
46 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
reproduction
Remarks on result:
other: A. caliginosa
Dose descriptor:
EC10
Effect conc.:
>= 120 mg/kg soil dw
Nominal / measured:
nominal
Basis for effect:
reproduction
Remarks on result:
other: E. crypticus
Details on results:
No toxicity to E. crypticus was seen at any dose level, including the highest dose level of 120 mg/kg soil. A dose-related inhibition of cocoon production was seen in A. caliginosa at the highest concentration of 150 mg/kg soil.

Applicant's summary and conclusion

Conclusions:
The EC10 for A. caliginosa was 46 mg/kg soil. The EC10 for E. crypticus was >=120 mg/kg soil.
Executive summary:

Groups of A. caliginosa were exposed to soil containing 25 -150 mg/kg soil of LAS for 28 days. Groups of E. crypticus were exposed to concentrations of 0, 10, 40, 60, 80, 100, or 120 mg/kg soil (nominal). EC10 values based on reproduction were 46 mg/kg soil for A. caliginosa, and >= 120 mg/kg soil for E. crypticus.